- ￥1900
- Biorigin
- BN40496R
- 2026年04月12日
- WB,
- Human,Mouse,Rat,Dog,Pig,Horse,Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Horse,Sheep,
- 应用范围：
WB,
- 抗体英文名：
CBX4
- 规格：
100ul
| 英文名称 | CBX4 |
| 中文名称 | 染色盒同源物4抗体 |
| 别 名 | CBX 4; CBX4; Cbx4 chromobox homolog 4 (Drosophila Pc class); CBX4_HUMAN; Chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox homolog 4; Chromobox protein homolog 4; E3 SUMO protein ligase CBX 4; E3 SUMO protein ligase CBX4; E3 SUMO-protein ligase CBX4; hPc 2; hPc2; NBP 16; NBP16; NS5ATP1 binding protein 16; Pc 2; Pc class 2 homolog; Pc class homolog; Pc class homolog Drosophila; PC2; Polycomb 2 homolog. |
| 研究领域 | 细胞生物 结合蛋白 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CBX4:401-500/560 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Protamines are small, arginine-rich (basic), nuclear proteins that mediate normal sperm head condensation and DNA stabilization (1–3). Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine (3,4). The majority of DNA in human sperm is bound to protamines with only a small proportion of DNA bound to histones in a way similar to active chromatin (1,2). The retention of histone association with sperm DNA with respect to protamine association to sperm DNA can change within as little as 400 bp of DNA, suggesting that there is fine control over haploid DNA organization (1). Protamines eventually replace histones late in the haploid phase of spermatogenesis (1,2). The human protamine 1 gene maps to chromosome 16p13.13 and encodes a 51 amino acid protein (5). The human protamine 2 gene maps to chromosome 16p13.13 and encodes a 102 amino acid protein (6). Function: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Subunit: Interacts with histone H3-K9Me3. Interacts with CHTOP (By similarity). Component of a PRC1-like complex. Self-associates. Interacts with SUV39H1 and HIPK2. Interacts with CSNK2B. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494. Similarity: Contains 1 chromo domain. SWISS: O00257 Gene ID: 8535 Database links: Entrez Gene: 8535 Human Entrez Gene: 12418 Mouse Omim: 603079 Human SwissProt: O00257 Human SwissProt: O55187 Mouse Unigene: 268070 Mouse Unigene: 471550 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验, 018等）和非标记两种定量法。 其中SPIDER是PEAKS中基于序列标签的搜索工具，可处理从头测序误差和同源突变之间可能出现的重叠部分。它可通过自动且有效地结合从头序列标签和同源物，重建正确的肽序列。 SPIDER 重建正确的肽序列过程（来源：百泰派克） PEAKS软件中使用的一系列算法已经过修改配置到PEAKS AB软件中，成为用于自动单克隆抗体测序公认的首选方法。
的染色盒内，防止干燥。 2．洗片 倾去存留的荧光抗体，将切片浸入pH7.4或pH7.2PBS 中洗两次，搅拌，每次5min，再用蒸馏水洗1min，除去盐结晶。 3．用50%缓冲（0.5mol/L碳酸盐缓冲液pH9.0～9.5）甘油封固、镜检 4．对照染色 ①正常免荧光血清染色，如上法处理切片，结果应为阴性。②染色抑制试验（一步法）：将荧光抗体和未标记的抗体球蛋白或血清（相同）等量混合，如上法处理切片。结果应为阴性。为证明此种染色抑制不是由于荧光抗体
一、原理与意义免疫荧光组织（细胞）化学染色方法——直接法，是一种荧光抗体染色法。该方法比较简单，适合做细菌、螺旋体、原虫、真菌及浓度较高的蛋白质抗原如肾、皮肤的检查和研究。此法每种荧光抗体只能检查一种相应的抗原，特异性高而敏感性较低。二、操作流程1、染色：切片经固定后，滴加经稀释至染色效价如1：8或1：16的荧光抗体（如兔抗人γ-球蛋白荧光抗体或兔抗人IgG或IgA荧光抗体等），在室温或37℃染色30min，切片置入能保持潮湿的染色盒内，防止干燥。2、洗片：倾去存留的荧光抗体，将切片浸入pH
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