O型密封圈（Type 42.2Ti)

LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER

Ti (1 hr,40,000 rpm)or 42.2Ti (30 min,25,000 rpm)at 4℃. 2.Polymerize actin by adding KCl to 100 mM and MgCl2 to 2 mM.Incubate for 30-60 min at room temperature. 3.Centrifuge in a 50Ti rotor for 1 hr at 40,000 rpm,15℃,or 42.2Ti rotor for 1/2 hr at 25

LABELING ALPHA-ACTININ WITH IATR

with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove

LABELING MUSCLE ACTIN WITH IATR

1.Centrifuge in a 50Ti (1 hr,40,000 rpm)or 42.2Ti (30 min,25,000 rpm)at 4℃. 2.Measure concentration and dye/protein molar ratio.Dilute 1:40 with the dialysis buffer and read the OD at 555 nm. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 43,000