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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
DNase I, Cell Culture Grade
- 供应商:
上海汇谱仪器有限公司
- CAS号:
459130-BR
- 规格:
100 mg sterile
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文献和实验RNA Isolation From Animal tissue or cell culture
the collection tube in step 8 or step 9. If on-membrane DNase I digestion is desired, proceed step 8, otherwise go to step 9. 8. DNase I Digestion (Optional): this is point to start On-membrane DNase I digestion. (See detail procedure on page 14).
The Cell Environment (including types of culture medium)
transformed cell lines require more acid conditions pH (7.0 - 7.4). Regulation of pH is particularly important immediately following cell seeding when a new culture is establishing and is usually achieved by one of two buffering systems; (i) a "natural
. Resuspend cells in appropriate medium or buffer. Quantitate cell yield and viability. Seed cells for culture, if required. Perfusion procedures require special equipment and techniques
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