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文献和实验Microinjection of dsRNA into Mouse Oocytes and Early Embryos
: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003. INTRODUCTION This protocol describes how to introduce a double-stranded RNA (dsRNA) of choice into mouse oocytes or fertilized
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
is formed over the needle tip. (C ) The needle tip is lifted slightly to release the pressure on the epiblast, without losing the vitelline-membrane fold. (D ) The needle tip is moved to the left to penetrate through the vitelline membrane. Steps B through D
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
(eg., 2h) grow in 5% CO2, at 37 C on 1 DIV (approx 24h), add araC to 10 uM: 24 well: 10ul 500uM 35mm: 30 ul 500uM [araC: -80C box G5 (HD) or E1] 60mm: 60ul 500uM feed with new media (containing araC) on (3)-4 DIV: 1ml/3ml per 60mm 200ul/500ul per 24
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