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文献和实验SequalPrep Long PCR Kit with dNTPs
0.4 μl -- SequalPrep™ 10X Enhancer A or B 1–2 μl* 0.5X–1X SequalPrep™ Long Polymerase
) for 1 minute to elute DNA. 12) For genomic DNA clean up, repeat elution step 10-11 by adding another 15-20 ul Elution Buffer. 2. MicroElute™ DNA Clean-Up Vacuum/Spin Protocol Note: Please read through previous section before using
"Glassing Out" DNA Using the Geneclean Kit
a fresh razor blade and place into a clean Eppendorf tube. Use long-wave UV light for as short a time as possible to visualize the DNA. 2. Determine the approximate volume of the gel slice by weight. If the slice weighs more than 0.4 g
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