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文献和实验Isolation of Bacterial Cell Membranes Proteins Using Carbonate Extraction
for solubilisation. Incubation of cell membrane preparations with sodium carbonate solution enriches for cell membrane proteins by stripping away other loosely associated protein contaminants from membranes (e.g., ribosomes, elongation factors). With the aid
Periodate Treatment of Fixed Cell Plates
1. Reagents Sodium Borohydride (NaBH4) - 1 mg/ml Carbonate:Bicarbonate Buffer, pH 9.6 Sodium Periodate (NaIO4) - 1 mM, 2.14 mg/10 ml Acetate Buffer 10mM Acetate Buffer, pH 4.5 0.82 g sodium acetate in 90 ml diH2O. Adjust to pH
.7)Rinse with water for 1 min.2X.0.1% Silver Nitrate and 0.08% Formalin (37%): For 1 gel,add 150 mg silver nitrate and 120μl 37% formalin to 150ml water.Developing: Must make fresh solution8)Incubate with 150ml 2% sodium carbonate with 0.04% formalin (37
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