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文献和实验Differential cDNA Screening Procedures
Differential cDNA Screening Procedures The protocols listed refer to cDNA library construction and preliminary differential screening procedures. They are mainly adaptations of standard procedures as per Sambrook et al. (1989
1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled with LB agar, stabbed with a toothpick which has been put into a bacterial colony). These are then frozen; at -70℃ they will keep indefinitely, at -20℃ for a year
全细胞靶点筛选抗生素新药的方法A target-specific whole cell assay for antibacterial drug discovery
that combines agar-diffusion and protein over expression techniques. This agar based two-plate differential sensitivity assay was used to help confirm the newly discovered antibiotic platensimycin inhibited bacterial growth by specifically targeting
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