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文献和实验Visualization of Live Primary Cilia Dynamics Using Fluorescence Microscopy
., Pypaert, M., Cantley, L., Toomre, D., and Mellman, I. 2007. Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells. J. Cell Biol. 179:1133‐1140.
were significantly decreased. High expressions of Bcl-2 and Bax were detected in 1 and 2μmol/L arseni c trioxide-treated cells, these were 46%, 87.33% and 83.08%, 95.83% respecti vely, among which that of Bax was more significant. Arsenic trioxide treatment at 0.5μ
Flow Cytometric Analysis Of Bcl Family members
Description Cell Fixation, staining and flow cytometric analysis Procedure Cells (106) were washed twice in FACS buffer (phosphate buffered saline PBS pH 7.4 containing 2% fetal calf serum(FCS) and 0.02% NaN3) and resuspended in 250ƒY´l
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