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文献和实验How to prepare Molecular Biology grade glycogen
Overview Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage
LC-MS/MS Screen for Xenobiotics and Metabolites
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is now considered as a perfect complement to HPLC-DAD (diode array detection) and gas chromatography (GC)-MS for the general unknown screening of drugs and toxic compounds
Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System
. Here we describe methods for the clinical grade isolation of blood DC and DC precursors using the CliniMACS™. We describe the isolation of ultra-pure monocytes in order to generate large numbers of monocyte-derived DC, and also new methods for the direct isolation of blood
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