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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Recombinant Human MDP1/Magnesium-dependent phosphatase 1
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥800.0 |
| 规格: | 500ug | 产品价格: | 询价 |
| 规格: | 1mg | 产品价格: | 询价 |
Recombinant Human MDP1/Magnesium-dependent phosphatase 1 (CI19)
产品说明(Description)
Recombinant Human Magnesium-dependent phosphatase 1 is produced by our Mammalian expression system and the target gene encoding Met1-Ala176 is expressed with a 6His tag at the C-terminus.
Accession #: Q86V88
Known as: Magnesium-dependent phosphatase 1;MDP-1;EC=3.1.3.48;
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10%Glycerol,pH7.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
MDP1, which is short for nesium-dependent phosphatase 1, is a 176 aa. protein. It has 3 forms by different alternative splicing. This protein belongs to the HAD-like hydrolase superfamily, and usually takes its function with Magnesium. It can be inhibited by vanadate and zinc, and slightly by calcium. MDP1 is a Magnesium-dependent phosphatase which may act as a tyrosine phosphatase.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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文献和实验Assaying Cdc25 Phosphatase Activity
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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