Recombinant Mouse GZMD/Granzym

Recombinant Mouse GZMD/Granzyme D (CM86)

 

产品说明(Description)

Recombinant Mouse Granzyme D is produced by our Mammalian expression system and the target gene encoding Ile21-Leu252 is expressed with a 6His tag at the C-terminus. 

Accession #: P11033

Known as: Granzyme D; Gzmd

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Granzyme D is a member of the granzyme family of the serine proteases which plays a role in the induction of apoptosis. T cells, lymphohematopoietic stromal cells, and granulated metrial gland cells express granzyme D, but the function of granzyme D is unknown. Previous studies reported that granzyme D is developmentally regulated during pregnancy together with granzymes E, F, and G in granulated metrial gland cells and is upregulated by IL-2 and IL-15. Granzyme D was also suggested to have a role in stromal cell-lymphocyte interactions.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY