Recombinant Human EIF1B/Eukary

Recombinant Human EIF1B/Eukaryotic Translation Initiation Factor 1B (C220)

 

产品说明(Description)

Recombinant Human Eukaryotic Translation Initiation Factor 1B is produced by our E.coli expression system and the target gene encoding Met1-Phe113 is expressed with a 6His tag at the N-terminus. 

Accession #: O60739

Known as: Eukaryotic Translation Initiation Factor 1b; eIF1b; Protein Translation Factor SUI1 Homolog GC20; EIF1B

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 500mM NaCl, pH 8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Eukaryotic Translation Initiation Factor 1B (EIF1B) is an element of a complex involved in recognition of the initiator codon during the scanning process. Translation is also initiated by the function of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. EIF1B enables 43S ribosomal complexes to distinguish between cognate and near-cognate initiation codons, perceiving the nucleotide content of initiation codons.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY