Recombinant Human CRYAA/Alpha-

Recombinant Human CRYAA/Alpha-crystallin A  (CF01)

产品说明(Description)

Recombinant Human alpha-Crystallin A Chain is produced by our E.coli expression system and the target gene encoding Met1-Ser173 is expressed with a 6His tag at the C-terminus. 

Accession #: P02489

Known as: Alpha-Crystallin A Chain; Heat Shock Protein Beta-4; HspB4; Alpha-Crystallin A Chain; Short Form; CRYAA; CRYA1; HSPB4

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, 2mM EDTA, pH 8.0.

 

质量控制(Quality Control) 

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Alpha-Crystallin A Chain (CRYAA) belongs to the small heat shock protein (HSP20) family and can be induced by heat shock. The expression of CRYAA is preferentially restricted to the lens cell. CRYAA may contribute to the transparency and refractive index of the lens. CRYAA has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. Two additional functions of CRYAA are an autokinase activity and participation in the intracellular architecture.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY