Recombinant Streptomyces hygro

Recombinant Streptomyces hygroscopicus BAR/Phosphinothricin N-acetyltransferase (CM65)

 

产品说明(Description)

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed. 

Accession #: P16426

Known as: Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY