Recombinant Cynomolgus TIGIT (

Recombinant Cynomolgus TIGIT (C-Fc) (CW11)

产品说明(Description)

Recombinant Cynomolgus T cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a Fc tag at the C-terminus. 

Accession #: G7NXM4

Known as: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9
 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS,pH 7.4.
 

 

质量控制(Quality Control) 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity-BLI:   

 

Loaded Human PVR-His(Cat#C622) on HIS1K Biosensor, can bind Cynomolgus TIGIT-Fc(Cat#CW11) with an affinity constant of 24.6 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Dissolve the lyophilized protein in distilled water.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY