Recombinant Human SIRPA (C-mIg

Recombinant Human SIRPA (C-mIgG2aFc) (CI33)

产品说明(Description)

Recombinant Human Signal-Regulatory Protein alpha-1 is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a mFc tag at the C-terminus. 

Accession #: CAA71403.1

Known as: Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1; Signal-regulatory protein alpha-1; p84; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.

 

质量控制(Quality Control)

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

 

 

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Human SIRPA-mFc(Cat#CI33) at 10μg/ml (100 μl/well) can bind Anti-Human SIRPA mAb-Fc(Cat#NC015). The ED50 of Anti-Human SIRPA mAb-Fc(Cat#NC015) is 47.9 ng/ml.		Loaded Anti-Human SIRPA mAb-Fc(Cat#NC015) on AHC Biosensor, can bind Human SIRPA-mIgG2aFc(Cat#CI33) with an affinity constant of 0.14 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY