Recombinant Human CD24 (C-Fc)

Recombinant Human CD24 (C-Fc)(C05B)

产品说明(Description)

Recombinant Human Signal Transducer CD24 is produced by our Mammalian expression system and the target gene encoding Ser27-Gly 59 is expressed with a Fc tag at the C-terminus.

Accession #: P25063

Known as:  Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24; CD24A 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity-Elisa:

 

Immobilized Anti-Human CD24 mAb(Cat#NC050) at 2μg/ml (100 μl/well) can bind Human CD24-Fc*(Cat#C05B)*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human CD24-Fc*(Cat#C05B) is 7.62 ng/ml.	000000	Immobilized Anti-Human CD24 mAb(Cat#NC050) at 2μg/ml (100 μl/well) can bind Human CD24-Fc*(Cat#C05B)*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human CD24-Fc*(Cat#C05B) is 7.62 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months. 

 

背景(Background)

Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs. 

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY