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- 2026年01月29日
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ANTI-RAD50 clone 13B3/2C6, 100ug
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文献和实验Single Clone Excision Protocol
500ml 20% maltose and 500ml 1 M MgSO4 to 50 ml LB broth). Next day: 3.If you're making new cells,spin them down 5 min at 1500 x g in the C0650 rotor in the Beckman centrifuge,then resuspend in about 40 ml 10 mM MgSO4,and quantify absorbance
Clone Genes From a Phage Library
). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition to the phage is: Plates: LB
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
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