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Anti-CD11c (mouse), Biotin, clone N418
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文献和实验Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
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