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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
TLK2 Antibody (Center)
- 抗体英文名:
TLK2 Antibody (Center)
- 应用范围:
WB
- 适应物种:
H M
- 克隆性:
多克隆
- 规格:
100UL
WBPrimary AccessionQ86UE8Other AccessionQ9UKI7ReactivityHuman, MouseIsotypeRabbit IgCalculated MW87661 DaTLK2 Antibody (Center) - Additional infoGene ID11011Other Names
TLK2; Serine/threonine-protein kinase tousled-like 2; PKU-alpha; Tousled-like kinase 2Target/Specificity
This TLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-171 amino acids from the Central region of human TLK2.Dilution
WB~~1:1000Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Precautions
TLK2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.TLK2 Antibody (Center) - Protein InformationNameTLK2Function
Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.Cellular Location
Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2Tissue Location
Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes
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文献和实验Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
(see Reagents and Solutions). 3- incubate with the blocking for 10 min. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking. 4- blot the slides without washing and apply the primary antibody, in a moist
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection. (on fixed, paraffin-embedded sections) Cut sections at 4µm, use a clean water bath with distilled water, and let the sections dry
with those with a postgerminal center/activated profile. Microarray technology may not be available in many diagnostic laboratories, and antibody-based methods are much simpler and cheaper and are therefore more applicable to the routine setting. Immunocytochemistry
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