Testosterone ELISA，唾液

Improved Saliva Testing of hormone levels

Enzyme immunoassay for the quantitative determination of free testosterone in human saliva.

The measurement of testosterone is used in the diagnosis and treatment of disorders involving the male sex hormones (androgens), including primary and secondary hypogonadism, and additionally: with impotence in males, in female hirsutism (excessive hair), virilization (masculinization) due to tumors, polycystic ovaries and adrenogenital syndromes.

The improved IBL International Testosterone Saliva ELISA is calibrated to the LC-MS/MS reference method and includes interchangeable reagents within the IBL saliva ELISA product line.
The Testosterone Saliva ELISA can easily be automated on open platforms like the EVOlyzer® from Tecan.

Benefits of the Testosterone Saliva ELISA

• Calibration to the LC-MS/MS
• Simplified pipetting protocol
• Optimization for automation
• Alignment to entire Saliva line
• High Sensitivity

ELISA vs. Mass Spectronomy

Mass spectrometry (MS) is considered as the reference method for steroid hormone quantification. This methodology does however represent a large investment and as such is not available to all laboratories. All the IBL saliva assays have been developed to correlate to this reference method, and even more importantly, only require low sample volume for quantification of the steroid hormones.

Consistent and reliable processing within Saliva product line

Our Saliva ELISA product line (Cortisol, Testosterone, Progesterone, DHEA) can be processed in a similar way and be easily performed in combination:

• The TMB, washing and stop solutions are interchangeable;
• The enzyme conjugate, the substrate and stop solutions are uniformly pipetted with 100 µL;
• The kits contain as few as 6 standards and 2 controls;
• Only 50 µL sample volume are required;
• All kits use the same sample diluent buffer.

Automation

The IBL International Testosterone Saliva ELISA (RE52631) can be adapted on different ELISA processors. Open automated platforms like the EVOlyzer® from Tecan have been tested and show good correlation to the manual runs:

Applications of Free Testosterone determination

Testosterone is a steroid hormone, produced in the testes of males and in smaller quantities in the ovaries of women. In both men and women a substantial amount is produced by the conversion of circulating DHEA-S, DHEA and androstenedione. It shows a diurnal rhythm, with highest levels in the morning and a nadir around midnight.

Testosterone plays an important role in the development of male reproductive tissues, supports increased muscle, bone mass, and hair growth and is essential for health, stamina, sexual function, cardiovascular health, and a healthy immune system.

The measurement of testosterone is used in the diagnosis and the treatment of disorders involving androgens, the male sex hormones.

These include primary and secondary hypogonadism, disorders originating from testicular problems or defects of the hypothalamus or pituitary glands. Furthermore with impotence in males, in female hirsutism (excessive hair), virilization (masculinization) due to tumors, polycystic ovaries and adrenogenital syndroms. Testosterone together with Cortisol levels also represents a useful parameter in stress research and sports medicine.

Measurement of Testosterone in saliva

Only 1-2% of the hormone Testosterone is unbound from plasma proteins and therefore biologically active. It is released via the salivary glands and mainly transformed to 5a-dihydrotestosterone. The concentration of testosterone in saliva, reflects however the level of free testosterone in plasma.

Pulsatile dynamics

Due to the pulsatile dynamics of Testosterone secretion repeated saliva sampling is recommended. We recommend collecting 3 to 5 saliva samples within 2 hours. In the laboratory, equal volumes of the individual saliva samples can be mixed. This mixed sample results in a mean free Testosterone value, which represents the active hormone concentration in a reproducible way.

Therefore measuring the free testosterone in saliva is a convenient non-invasive method. Easier sample collection without repeated venipunctures and reduced stress on the specimen offer benefits to the patient while sensibility and accuracy of the results are ensured.