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- 2026年01月25日
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- 库存：
50
- 供应商：
广州市左克生物
- CAS号：
37288-25-8
- 规格：
10KU
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文献和实验Detection of Viral microRNA with S1 Nuclease Protection Assay
and quantification of these molecules. Here, we present a simple, specific, and very sensitive protocol using short radioactive DNA oligonucleotides for hybridization to homologous RNA target in a nuclease protection assay. The S1 nuclease from Aspergillus oryzae
S1 Nuclease Protection Mapping
The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp (1 ), it has undergone many refinements over the years. S1 nuclease mapping requires a relatively
4. Vortex vigorously. 5. Incubate at 100 deg C for 3 minutes. 6. Transfer immediately to 58 deg C and incubate overnight. S1 digestion buffer (final concentration): 300 mM NaCl 30 mM sodium acetate, pH 5.5 2 mM zinc sulfate 1000 units/ml S1 nuclease
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