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货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
37288-25-8
- 规格:
10 KU
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Nuclease S1
CAS No. : 37288-25-8
MCE 国际站:Nuclease S1
产品活性:Nuclease S1 是一种特异性的核酸内切酶,可降解单链 DNA (ssDNA) 和 RNA。Nuclease S1 还可以去除双链 DNA 中突出的单链末端。
研究领域:Cell Cycle/DNA Damage
作用靶点:DNA/RNA Synthesis
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文献和实验digested plasmid (cut 5 µg of plasmid in volume of 20 µl) 51 µl labeled oligo 10 µl 10X Taq Buffer 6 µl 25 mM MgCl2 10 µl DMSO 2 µl 10 mM dNTP's 1 µl Taq polymerase 14 cycles of PCR Run on 6-8% polyacrylamide/urea gel Cut out probe and elute S1
S1核糖核酸酶保护分析实验(S1 Nuclease Protection Assay)
相关专题 核糖核酸酶保护实验 S1 Nuclease Protection Assay End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + oligo (200 ng) 1 µl T4 Kinase 1. 37
Detection of Viral microRNA with S1 Nuclease Protection Assay
and quantification of these molecules. Here, we present a simple, specific, and very sensitive protocol using short radioactive DNA oligonucleotides for hybridization to homologous RNA target in a nuclease protection assay. The S1 nuclease from Aspergillus oryzae
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