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Mouse Branched-chain-amino-aci

d aminotransferase, Mitochondrial (BCAT2) ELISA Kit试剂盒,orb1146805,biorbyt
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  • ¥3900
  • biorbyt已认证
  • 15.63-1000 pg/mL
  • 6.3 pg/mL
  • orb1146805
  • 英国
  • 2026年01月22日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      15.63-1000 pg/mL

    • 检测方法

      Sandwich

    • 适应物种

      Mouse

    • 样本

      serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

    • 灵敏度

      6.3 pg/mL

    • 规格

      48 T

    产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2) in the samples is then determined by comparing the OD of the samples to the standard curve.

    产品别名:Branched-chain-amino-acid aminotransferase, mitochondrial, BCAT(m), Bcatm, Eca40, Bcat2

    应用笔记:standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BCAT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BCAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCAT2 in the samples is then determined by comparing the OD of the samples to the standard curve

    实验时长:3.5h

    UniProt ID:O35855

    Note:For research use only.

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    图标文献和实验
    相关实验
    • 2003年国际主要分子生物学数据库

      for mitochondrial proteins 117.  MITOP http://www.mips.biochem.mpg.de/proj/medgen/mitop/ Mitochondrial proteins, genes and diseases 118.  MOsDB http://mips.gsf.de/proj/rice Oryza sativa genome 119.  Mouse Genome Database (MGD) http://www.informatics.jax.org

    • [资源] 所有的看家基因(housekeeping genes)列表+引物设计服务

      1283 NM_002568 Homo sapiens poly(A) binding protein, cytoplasmic 1 (PABPC1), mRNA 3199 NM_001487 Homo sapiens GCN5 general control of amino-acid synthesis 5-like 1 (yeast) (GCN5L1), mRNA 313 NM_001861 Homo sapiens cytochrome c oxidase subunit

    • Analysis of Oxidative Modification of Proteins

      between protein molecules and reactive oxygen species (ROS) often lead to the modification of certain amino acid residues such as histidine, lysine, arginine, proline, and threonine, forming carbonyl derivatives. Carbonylation of proteins

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