Mouse Branched-chain-amino-aci

产品描述：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.

产品别名：Branched-chain-amino-acid aminotransferase, cytosolic, BCAT (c) , Protein ECA39, Eca39, Bcat1

应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BCAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BCAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCAT1 in the samples is then determined by comparing the OD of the samples to the standard curve

实验时长：3.5h

UniProt ID:P24288

Note：For research use only.