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- 详细信息
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Sheep
- 样本:
serum, plasma and other biological fluids
- 灵敏度:
0.056 ng/mL
- 规格:
48 T
产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy associated glycoprotein 1(PAG1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnancy associated glycoprotein 1(PAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy associated glycoprotein 1(PAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy associated glycoprotein 1(PAG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
产品别名:PAG1, Pregnancy associated glycoprotein 1
应用笔记:standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep PAG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep PAG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep PAG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep PAG1 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:Q28755
Note:For research use only.
产品别名:PAG1, Pregnancy associated glycoprotein 1
应用笔记:standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep PAG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep PAG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep PAG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep PAG1 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:Q28755
Note:For research use only.
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Sheep Pregnancy Associated glycoprotein 1 (PAG1) ELISA Kit试剂盒,orb1146844,biorbyt
¥3900






