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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
7.82-500 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 灵敏度:
3 pg/mL
- 规格:
48 T
产品别名:IL-27, p28, IL30, Interleukin 30, Interleukin-27 subunit alpha
应用笔记:standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL30 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
Note:For research use only.
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文献和实验ELISAs and Interleukin Research
and biomedical research; it has been modified and adapted for multiple applications since its development almost 30 years ago (1). As the most commonly used immunoassay technique, ELISA provides the basis for numerous tests in the study of infectious diseases
人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本 中Neuritin 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 Neuritin ,再与 HRP 标记的 Neuritin 抗体结合,形成抗体
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important






