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Human Soluble Interleukin 30 (

sIL30) ELISA Kit试剂盒,orb1088138,biorbyt
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  • ¥3900
  • biorbyt已认证
  • 7.82-500 pg/mL
  • 3 pg/mL
  • orb1088138
  • 英国
  • 2026年01月22日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      7.82-500 pg/mL

    • 检测方法

      Sandwich

    • 适应物种

      Human

    • 样本

      Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

    • 灵敏度

      3 pg/mL

    • 规格

      48 T

    产品描述:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Interleukin 30(sIL30). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Interleukin 30(sIL30). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Interleukin 30(sIL30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Interleukin 30(sIL30) in the samples is then determined by comparing the OD of the samples to the standard curve.

    产品别名:IL-27, p28, IL30, Interleukin 30, Interleukin-27 subunit alpha

    应用笔记:standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL30 in the samples is then determined by comparing the OD of the samples to the standard curve

    实验时长:3.5h

    Note:For research use only.

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