ELISAs and Interleukin Research

ELISA (enzyme-linked immunosorbent assay) is a powerful, versatile, precise, and reliable quantitative technique for the measurement of antigens or antibodies in biologic samples. The ELISA technique is a widely used tool in biologic and biomedical research; it has been modified and adapted for multiple applications since its development almost 30 years ago (1). As the most commonly used immunoassay technique, ELISA provides the basis for numerous tests in the study of infectious diseases, epidemiology, endocrinology, and immunology. The development of specific ELISA antibodies and reagents has helped to revolutionize the field of immunology, in particular by providing a simple, rapid, and reproducible method of evaluating the role of immune mediators in disease processes.