Human Eukaryotic Translation I
nitiation Factor 2 Alpha Kinase 3 (EIF2aK3) ELISA Kit试剂盒，orb866890，biorbyt- ￥3900
- biorbyt
- 0.16-10 ng/mL
- 0.062 ng/mL
- orb866890
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Human
- 样本：
Tissue homogenates and other biological fluids.
- 灵敏度：
0.062 ng/mL
- 规格：
48 T
产品别名：WRS ELISA Kit, PEK ELISA Kit, PERK ELISA Kit, HsPEK ELISA Kit, PRKR-like endoplasmic reticulum kinase ELISA Kit, Pancreatic eIF2-alpha kinase ELISA Kit
应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF2aK3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF2aK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF2aK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF2aK3 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q9NZJ5
Note：For research use only.
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文献和实验interferon response elements in the promoter region, which are required for binding of the interferon-stimulated gene factor 3 (ISGF3) complex and for janus kinase/signal transducer of transcription (JAK/STAT)-pathway-mediated signaling. Expression of PRGs
Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling
PKR in Innate Immunity, Cancer, and Viral Oncolysis
found in most human cells. Following activation by double stranded (ds) RNAs produced during viral replication, PKR phosphorylates the α-subunit of eukaryotic translation initiation factor (eIF) 2, causing a severe inhibititon of cellular and viral protein synthesis
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