- ￥3900
- biorbyt
- 1.57-100 ng/mL
- 0.57 ng/mL
- orb866907
- 英国
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
1.57-100 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度：
0.57 ng/mL
- 规格：
48 T
产品别名：[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial ELISA Kit
应用笔记：standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDK1 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:Q15118
Note：For research use only.
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文献和实验gene，外膜孔蛋白 ORF ，open reading frame，开放阅读框 ori ，origin，复制起始点 PCR ，Polymerase chain reaction，多聚酶链反应 PDH ，pyruvate dehydrogenase，丙酮酸脱氢酶 PEG ，Polyethyleneglycol 聚乙二醇 pfu ，plaque forming units，空斑形成单位 PAGE ，polyacrymide gel
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
botulinum neurotoxin serotype FI, 240 mg/kg cells307Clostridium botulinum neurotoxin serotype A binding domainI, 2.4 mg total[59]Clostridium tetani Tetanus toxin fragment CI, 12 g/L[60]Diphtheria anti-human anti-T cell immunotoxinS, 10 mg/L, α-MF
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