- ￥3900
- biorbyt
- 31.25-2000 pg/mL
- 12 pg/mL
- orb776786
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
31.25-2000 pg/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Rabbit
- 样本：
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
12 pg/mL
- 规格：
48 T
产品别名：HCNTF ELISA Kit
应用笔记：standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit CNTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CNTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit CNTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CNTF in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P14188
Note：For research use only.
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文献和实验Expression of Neurotrophic Factors such as GDNF in the E. coli System
neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial-cell-line-derived neurotrophic factor (GDNF), ciliary neurotrophic factor (CNTF), insulin-like growth factor-1 (IGF-I), are currently in clinical trials and have potential
Identification of Neurotrophic Factors by Molecular Biological Techniques
factor (BDNF), and ciliary neurotrophic factor (CNTF) were discovered by their specific neurotrophic properties. They were purified from tissue extracts using in vitro bioassays based on the survival of specific nerve cells and subsequently
2个HCRF结构域 LIFR/gp130 20～30pM OSM gp130 1个HCSF结构域 500pM gp130/LIFR 5pM CNTF CNTFRα链 1个ICSF结构域 10nM CNTFRα/gp130、LIFR 100～200pM 1个HCSF结构域（GPI锚） 注：（1）LIF：leukaemia inhibitory factor;OSM:oncostatin M;CNTF:ciliary neurotrophic factor. (2)IGSF
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