- ￥3900
- biorbyt
- 15.63-1000 pg/mL
- 6 pg/mL
- orb776288
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Rabbit
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
6 pg/mL
- 规格：
48 T
产品别名：IFN gamma,IFN-G ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, INFr ELISA Kit, IFN, Immune Interferon ELISA Kit
应用笔记：standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IFNg in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P30123
Note：For research use only.
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文献和实验a stereomicroscopeReferenceszerkinsky et al., A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods 1983; 65: 109.Asai et al., Evaluation of the modified ELISPOT assay for gamma interferon production in cancer
ELISPOT (Enzyme-linked ImmunoSPOT)
for detection of antigen-specifi c memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods. 285: 223-235. Smith JG, et al. 2001. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
39611β-HSD 1B variantI, active548.0Human serum albuminS, 13.4mg/g biomass536InsulinS, synthetic signal or α-MF, 1.5 g/L, active[233], 532Insulin-like growth factor-1 (IGF-1)S, 600 mg/L, α-MF[234Interferon α-1S, α-MF, 35 mg/L, active520Interferon gamma
