- ￥3900
- biorbyt
- 0.16-10 ng/mL
- 0.064 ng/mL
- orb776101
- 英国
- 2026年01月21日
企业认证
相关产品推荐更多 >
Human Glycogen Phosphorylase, Liver (PYGL) ELISA Kit试剂盒，orb1949053，biorbyt
￥3770
Human Secretory Carrier-Associated Membrane Protein 3(SCAMP3) ELISA Kit 酶联免疫试剂盒，orb1909639，Biorbyt
￥6968
Human IgA ELISA KIT 酶联免疫试剂盒，orb1086078，Biorbyt
￥4664
Human TIE2 ELISA Kit试剂盒，orb391012，biorbyt
￥8580
Human Nephronectin (NPNT) ELISA Kit试剂盒，orb777062，biorbyt
￥3900
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Mouse
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
0.064 ng/mL
- 规格：
48 T
产品别名：B4 ELISA Kit, B-lymphocyte surface antigen B4 ELISA Kit, T-cell surface antigen Leu-12 ELISA Kit
应用笔记：standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD19. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD19. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD19, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD19 in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
UniProt ID:P25918
Note：For research use only.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
127+ ) from the bone marrow or thymus or more downstream progenitors such as pre-B- (CD117+ CD19+ ) or pre-T-cells (CD44+ CD25+ CD3� CD4� CD8� ) are used, then the differentiation kinetics are accelerated from those shown in Figures 4 and 5 . ACKNOWLEDGMENTS
.: +33-4-77-81-4366; fax: +33-4-77-80-8294.Manuscript received 11 May 2003 Accepted 20 July 2003;Article OutlineAbstractIntroductionMaterials and methodsCell preparationsCell stimulationDetection of NFB translocation by flow cytometric assay in CD19+ B
Intracellular Cytokine Staining Protocol
immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinMP6-XT22TNF-aNEW!mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinTN3-19.12Annotations: mouse PEC=mouse thioglycolate
