- ￥3770
- biorbyt
- 0.16-10ng/mL
- 0.1 ng/mL
- orb1949053
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.16-10ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.1 ng/mL
- 规格：
48 T
产品别名：Hers disease, glycogen storage disease type VI, GSD6
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYGL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PYGL. You can calculate the concentration of Human PYGL in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P06737
靶点：PYGL
Note：For research use only.
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途径产生 NADPH，NADPH 维持细胞内氧化还原稳态，保证 Tm 细胞二次激活反应时的高质量和高活性。 糖原磷酸化酶（Glycogen phosphorylase，PYG）是糖原分解反应的关键酶，催化糖原裂解为 1 磷酸葡萄糖，具有三种亚型（PYGL，Liver；PYGM， Muscle；PYGB， Brain）。本研究发现 PYGB 在 T 细胞中高表达。 重要的是，PYG 一直被认为通过其丝氨酸磷酸化而发挥活性功能；本研究还首次发现 TCR 的下游信号分子 ZAP70/LCK 能够
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