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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
0.32-20ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度:
0.19 ng/mL
- 规格:
48 T
产品别名:PDP
应用笔记:This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDP. You can calculate the concentration of Human PDP in the samples by comparing the OD of the samples to the standard curve.
实验时长:3.5H
UniProt ID:Q9P0J1
靶点:PDP
Note:For research use only.
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文献和实验of pyruvate dehydrogenase. We then went on to validate these findings in a comprehensive biochemical study. Consistent with increased phosphorylation of Hxk2p in response to REG1 deletion, fractionation of yeast extracts by anion-exchange chromatography
gene,外膜孔蛋白 ORF ,open reading frame,开放阅读框 ori ,origin,复制起始点 PCR ,Polymerase chain reaction,多聚酶链反应 PDH ,pyruvate dehydrogenase,丙酮酸脱氢酶 PEG ,Polyethyleneglycol 聚乙二醇 pfu ,plaque forming units,空斑形成单位 PAGE ,polyacrymide gel
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
from the article "Human Housekeeping genes are compact," published in Trends in Genetics 19, 362-365 (2003). Each gene name/description is followed by its geometric average expression level according to the data published by Su et al. Genes designated by asterisk







