肌酸激酶MB同工酶酶联免疫试剂盒(小鼠) CKMB/CK2/

Mouse Creatine Kinase Mb Isoenzyme (CK-MB) ELISA Kit (One Step Method)

小鼠肌酸激酶MB同工酶酶联免疫试剂盒 (一步法)

货号: KTE4073-3

产品分类: 核心产品 - 细胞分析

反应性: 小鼠

检测方法: 双抗体夹心法 ELISA

产品概述

本试剂盒采用一步法双抗体夹心ELISA原理，专为精准定量小鼠血清、血浆、组织匀浆及细胞上清液等样本中的肌酸激酶MB同工酶（CK-MB）水平而设计。该试剂盒是心血管疾病、肌肉损伤及相关生命科学研究领域的理想工具，尤其适用于小鼠模型中的心肌损伤评估。

核心特点与优势

• 高灵敏度: 最低检测限可达0.125 ng/mL，能够精准检测低丰度样本。
• 宽动态范围: 检测范围为0.625 ng/mL - 50 ng/mL，满足不同浓度样本的检测需求。
• 优异特异性: 基于EliKine™技术平台，经验证与目标蛋白的类似物无明显交叉反应，背景干扰低。
• 操作简便: 一步法操作流程，节省实验时间，减少操作误差。
• 即用型组分: 试剂盒提供预包被微孔板、即用型标准品和检测抗体，开盒即用，省去繁琐的准备工作。

检测原理

试剂盒采用双抗体夹心法。预包被在微孔板上的抗小鼠CK-MB抗体捕获样本中的目标蛋白，随后依次加入生物素化的检测抗体和链霉亲和素-HRP复合物，形成抗体-抗原-抗体-酶复合物。加入TMB底物显色后，颜色的深度与样本中CK-MB的浓度成正比，通过在450 nm波长下测定吸光度（OD值）并进行标准曲线计算，即可实现准确定量。

试剂盒组分

• 预包被小鼠CK-MB微孔板 (Mouse CK-MB Microplate)
• 小鼠CK-MB标准品 (100 ng/mL) (Mouse CK-MB Standard)
• 标准品稀释液 (Standard Diluent)
• 小鼠CK-MB检测抗体 (Mouse CK-MB Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A (HRP Substrate A)
• HRP底物B (HRP Substrate B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

样本类型

适用于小鼠的血清、血浆、组织匀浆、细胞培养上清液及相关液体样本。

规格与价格

规格	价格 (人民币)	货期
48 T	1,998.0	期货（3天内发货）
96 T	3,198.0	期货（3天内发货）

保存与运输

保存建议: 未开封的试剂盒请避光保存于2-8℃，有效期6个月。启用后，请参照说明书分别保存各组分。

运输条件: 全程采用蓝冰冰袋运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对标准品和待测样品进行复孔（双孔或三孔）检测。

警告: 本产品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

别名信息

CKMB; CK2; CK-MB; Creatine Kinase, Muscle/Brain

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Creatine Kinase Mb Isoenzyme (CK-MB) ELISA Kit (One Step Method)

Mouse Creatine Kinase MB Isoenzyme ELISA Kit (One Step Method)

Catalog Number: KTE4073-3

Product Category: Core Products - Cell Analysis

Reactivity: Mouse

Assay Method: Sandwich ELISA

Product Overview

This kit employs a one-step sandwich ELISA principle and is specifically designed for the accurate quantification of Creatine Kinase MB Isoenzyme (CK-MB) levels in mouse samples such as serum, plasma, tissue homogenates, and cell culture supernatants. It is an ideal tool for cardiovascular disease, muscle injury, and related life science research, particularly for the assessment of myocardial injury in mouse models.

Key Features & Advantages

• High Sensitivity: Minimum detectable limit as low as 0.125 ng/mL, enabling precise detection of low-abundance samples.
• Wide Dynamic Range: Detection range from 0.625 ng/mL to 50 ng/mL, meeting the detection needs of samples with varying concentrations.
• Excellent Specificity: Based on the EliKine™ technology platform, validated to show no significant cross-reactivity with analogs of the target protein and low background interference.
• Simple Operation: One-step procedure saves experimental time and reduces operational errors.
• Ready-to-Use Components: The kit provides pre-coated microplates, ready-to-use standards, and detection antibodies, allowing for immediate use and eliminating tedious preparation steps.

Assay Principle

The kit utilizes a sandwich ELISA method. The anti-mouse CK-MB antibody pre-coated on the microplate captures the target protein from the sample. Subsequently, a biotinylated detection antibody and a Streptavidin-HRP conjugate are added sequentially, forming an antibody-antigen-antibody-enzyme complex. After adding TMB substrate for color development, the color intensity is proportional to the concentration of CK-MB in the sample. Accurate quantification is achieved by measuring the absorbance (OD value) at 450 nm and calculating against a standard curve.

Kit Components

• Pre-coated Mouse CK-MB Microplate
• Mouse CK-MB Standard (100 ng/mL)
• Standard Diluent
• Mouse CK-MB Detect Antibody
• Streptavidin-HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

Sample Types

Suitable for mouse serum, plasma, tissue homogenates, cell culture supernatants, and related liquid samples.

Specifications & Price

Specification	Price (RMB)	Delivery Time
48 T	1,998.0	Stock (Ships within 3 days)
96 T	3,198.0	Stock (Ships within 3 days)

Storage & Shipping

Storage Recommendation: Store the unopened kit protected from light at 2-8°C; it is stable for 6 months. After first use, store components separately according to the instructions.

Shipping Conditions: Shipped with blue ice packs to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, use disposable pipette tips throughout the assay procedure.
• Unused strips should be immediately returned to the original pouch and sealed for storage at 4°C.
• Thorough mixing is crucial for reliable results; use a low-speed shaker or periodic manual shaking is recommended.
• For more reliable data, it is recommended to assay standards and unknown samples in duplicate or triplicate.

Warning: This product is for research use only, not for human use or clinical diagnosis. Users should ensure their application does not violate any patents or licensing regulations.

Alias Information

CKMB; CK2; CK-MB; Creatine Kinase, Muscle/Brain

Note: The above information may be subject to change due to product optimization and upgrades. Please contact Yakenin Company for specific details.