干细胞因子酶联免疫试剂盒(小鼠) SCF ELISA KIT

小鼠干细胞因子(SCF)酶联免疫试剂盒 (Mouse SCF ELISA Kit)

货号: KTE3133-3 | 产品类型: EliKine™ 核心产品，夹心法ELISA试剂盒

产品概述

本试剂盒是一款高灵敏度的双抗体夹心法ELISA检测试剂盒，专门设计用于定量检测小鼠来源样本（如细胞培养上清、血清、血浆）中的干细胞因子(SCF)含量。该试剂盒凭借其优异的特异性和精准的检测性能，成为干细胞与免疫学研究领域的可靠工具。

核心特性与优势

• 高灵敏度: 最低检测浓度可达 7.81 pg/mL，能够精确测量低丰度样本。
• 优异特异性: EliKine™ 技术确保了对目标蛋白的高特异性识别，经严格验证，与类似物无明显交叉反应或背景干扰。
• 宽线性范围: 检测范围为 7.81 pg/mL - 500 pg/mL，满足绝大多数实验需求。
• 可靠的重复性: 优化的缓冲体系与稳定的酶标板包被工艺，保障了实验结果的可重复性。

试剂盒组分（详细列表）

• 预包被Mouse SCF微孔板 (Mouse SCF Microplate)
• 小鼠SCF标准品 (冻干) (Mouse SCF Standard, lyophilized)
• 检测缓冲液 (10×浓缩) (Assay Buffer, 10×)
• 小鼠SCF检测抗体 (100×浓缩) (Mouse SCF Detect Antibody, 100×)
• 链霉亲和素-HRP (100×浓缩) (Streptavidin-HRP, 100×)
• HRP底物 (TMB) (HRP Substrate)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×浓缩) (Wash Buffer, 20×)
• 板盖 (Plate Covers)

技术参数

反应种属	小鼠 (Mouse)
样本类型	细胞培养上清、血清、血浆
检测方法	夹心法ELISA
检测范围	7.81 - 500 pg/mL
灵敏度	7.81 pg/mL
保存条件	未开封试剂盒请避光保存于2-8°C，保质期24个月
运输条件	冰袋（蓝冰）运输

规格与定价

规格	价格 (元)	货期
48 T	1598.0	请咨询
96 T	2558.0	期货（5个工作日内发货）

背景信息

干细胞因子(SCF)是c-Kit受体的配体，以跨膜和可溶性两种形式存在。它在造血、细胞增殖、迁移及存活等关键生理过程中扮演重要角色，尤其在肥大细胞功能、精子发生和黑素生成中作用显著。研究表明，SCF与多种生长因子协同作用，影响髓系和红系造血祖细胞的发育，并在心肌修复等病理过程中具有潜在应用价值。

重要操作提示

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，建议使用一次性吸头。
• 未使用的板条应立即放回原包装并密封，于4°C保存。
• 实验过程中充分的混匀至关重要，推荐使用振荡器或定期手动摇匀。
• 为保证数据准确性，建议对样品和标准品进行复孔检测。

警告: 本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或法规。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Stem Cell Factor (SCF) ELISA Kit

Catalog Number: KTE3133-3 | Product Type: EliKine™ Core Product, Sandwich ELISA Kit

Product Overview

This kit is a highly sensitive, double-antibody sandwich ELISA detection kit specifically designed for the quantitative measurement of Stem Cell Factor (SCF) levels in mouse-derived samples (such as cell culture supernatants, serum, and plasma). With its excellent specificity and accurate detection performance, this kit serves as a reliable tool in stem cell and immunology research.

Core Features & Advantages

• High Sensitivity: Minimum detectable concentration reaches 7.81 pg/mL, enabling precise measurement of low-abundance samples.
• Excellent Specificity: EliKine™ technology ensures high specificity for the target protein, rigorously validated with no significant cross-reactivity or background interference with analogs.
• Wide Linear Range: Detection range is 7.81 pg/mL - 500 pg/mL, meeting the requirements of most experiments.
• Reliable Reproducibility: Optimized buffer system and stable microplate coating process ensure reproducible experimental results.

Kit Components (Detailed List)

• Pre-coated Mouse SCF Microplate
• Mouse SCF Standard (lyophilized)
• Assay Buffer (10× Concentrate)
• Mouse SCF Detect Antibody (100× Concentrate)
• Streptavidin-HRP (100× Concentrate)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20× Concentrate)
• Plate Covers

Technical Parameters

Reactivity Species	Mouse
Sample Types	Cell culture supernatant, serum, plasma
Detection Method	Sandwich ELISA
Detection Range	7.81 - 500 pg/mL
Sensitivity	7.81 pg/mL
Storage Conditions	Store the unopened kit protected from light at 2-8°C; shelf life is 24 months.
Shipping Conditions	Shipped with ice packs (blue ice).

Specifications & Pricing

Specification	Price (CNY)	Availability
48 T	1598.0	Please inquire
96 T	2558.0	Future goods (shipped within 5 business days)

Background Information

Stem Cell Factor (SCF) is the ligand for the c-Kit receptor and exists in both transmembrane and soluble forms. It plays important roles in key physiological processes such as hematopoiesis, cell proliferation, migration, and survival, with significant functions particularly in mast cell function, spermatogenesis, and melanogenesis. Research indicates that SCF acts synergistically with various growth factors, influencing the development of myeloid and erythroid hematopoietic progenitor cells, and holds potential application value in pathological processes such as myocardial repair.

Important Operational Notes

• Do not mix reagents from different lots or expired reagents.
• Before use, all reagents should be equilibrated at room temperature for approximately 30 minutes.
• To avoid cross-contamination, the use of disposable pipette tips is recommended.
• Unused microplate strips should be returned to the original packaging, sealed, and stored at 4°C immediately.
• Thorough mixing during the procedure is crucial; the use of a plate shaker or regular manual shaking is recommended.
• For data accuracy, it is recommended to perform duplicate measurements for both samples and standards.

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure their application does not violate relevant patents or regulations.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.