MMP-2/MMP2/CLG4/CLG4A/MMP-II/M

产品概览

英文名称：Mouse MMP-2 ELISA Kit (One Step Method)
中文名称：MMP-2/MMP2/CLG4/CLG4A/MMP‑II/MONA/TBE‑1/PEX/Gelatinase A ELISA KIT 基质金属蛋白酶‑2 ELISA 试剂盒（小鼠）
货号：KTE4035-2
适用物种：小鼠

核心应用领域

• 产品分类：核心产品
• 产品中心：细胞分析
• 一级应用：ELISA 应用
• 检测类型：ELISA（双抗体夹心法）
• 样本类型：血清、血浆、组织、细胞上清及其他相关液体样本

技术原理

本套件采用双抗体夹心 ELISA 法测定小鼠基质金属蛋白酶‑2（MMP‑2）水平：

1. 酶标板预包被特异性抗小鼠 MMP‑2 抗体。
2. 加入样本或标准品，使 MMP‑2 与包被抗体形成捕获复合物。
3. 加入生物素化检测抗体，随后加入链霉亲和素‑HRP，形成抗体‑抗原‑抗体‑酶复合物。
4. 经充分洗涤后加入 HRP 底物（TMB），在酶催化下显色。
5. 加入终止液后，颜色转为黄色，用酶标仪在 450 nm 波长读取 OD 值。
6. OD 与 MMP‑2 浓度呈正比，可通过标准曲线计算样本浓度。

主要性能参数

试剂盒组成

• Mouse MMP‑2 Microplate（预包被抗体）
• Mouse MMP‑2 Standard（240 ng/mL）
• Standard Diluent
• Mouse MMP‑2 Detect Antibody（生物素化）
• Streptavidin‑HRP
• HRP Substrate A（TMB）
• HRP Substrate B（终止液）
• Wash Buffer
• Plate Covers

操作要点

1. 使用前将所有试剂在室温（≈20‑25 °C）平衡 30 min。
2. 全程推荐使用一次性吸头，防止交叉污染。
3. 未使用的酶标板请立即放回原包装，密闭保存于 4 °C。
4. 混匀是关键：建议使用低速振荡器或每 10 min 手动轻轻摇匀。
5. 样品和标准品建议设置双孔或三孔（复孔），提高结果可信度。
6. 同一批次试剂请勿与其他批次或过期试剂混用。

存储与运输

• 未开封试剂盒：避光保存于 2‑8 °C，保质期 6 个月。
• 开封后：请参照说明书中各组分的具体保存要求。
• 运输方式：使用蓝冰（冰袋）冷链运输，确保全程低温。

规格与报价

法规信息与免责声明

本产品仅供科研使用，不适用于人类诊断或临床治疗。使用本套件进行的任何实验均需遵守所在机构的伦理与法规要求。我们不对因使用本产品而导致的专利侵权或其他法律责任承担任何责任。

关键字检索（方便搜索）

Matrix Metalloproteinase 2, MMP‑2, MMP2, CLG4, CLG4A, MMP‑II, MONA, TBE‑1, PEX, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse MMP-2 ELISA Kit (One Step Method)
Chinese Name: MMP-2/MMP2/CLG4/CLG4A/MMP‑II/MONA/TBE‑1/PEX/Gelatinase A ELISA KIT Matrix Metalloproteinase‑2 ELISA Kit (Mouse)
Part Number: KTE4035-2
Applicable Species: Mouse

Core Application Areas

• Product Category: Core Product
• Product Center: Cell Analysis
• Primary Application: ELISA Application
• Assay Type: ELISA (sandwich immunoassay)
• Sample Types: Serum, plasma, tissue, cell supernatant and other related liquid samples

Technical Principle

This kit employs a sandwich ELISA method to quantify mouse matrix metalloproteinase‑2 (MMP‑2) levels:

1. Microplate pre‑coated with a specific anti‑mouse MMP‑2 antibody.
2. Add sample or standard, allowing MMP‑2 to bind the coated antibody forming a capture complex.
3. Add biotinylated detection antibody, followed by streptavidin‑HRP, generating an antibody‑antigen‑antibody‑enzyme complex.
4. After thorough washing, add HRP substrate (TMB) for enzymatic color development.
5. Add stop solution; the color changes to yellow and OD is read at 450 nm on a microplate reader.
6. OD is proportional to MMP‑2 concentration; sample concentration is calculated using the standard curve.

Key Performance Specifications

Kit Components

• Mouse MMP‑2 Microplate (pre‑coated antibody)
• Mouse MMP‑2 Standard (240 ng/mL)
• Standard Diluent
• Mouse MMP‑2 Detect Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A (TMB)
• HRP Substrate B (stop solution)
• Wash Buffer
• Plate Covers

Critical Procedural Steps

1. Equilibrate all reagents at room temperature (≈20‑25 °C) for 30 min before use.
2. Use disposable pipette tips throughout to prevent cross‑contamination.
3. Return unused microplates to original packaging and store sealed at 4 °C.
4. Mixing is critical: recommend low‑speed orbital shaker or gentle manual inversion every 10 min.
5. Run samples and standards in duplicate or triplicate wells to enhance data reliability.
6. Do not mix reagents from different lots or expired reagents.

Storage and Shipping

• Unopened Kit: Store protected from light at 2‑8 °C; shelf life 6 months.
• After Opening: Refer to the user manual for specific storage conditions of each component.
• Shipping Method: Ship on cold chain using blue ice packs to maintain low temperature throughout.

Specifications and Pricing

Regulatory Information and Disclaimer

This product is intended for research use only and is not suitable for human diagnostic or clinical therapeutic applications. All experiments performed with this kit must comply with the ethical and regulatory requirements of the respective institution. We assume no responsibility for patent infringement or any other legal liabilities arising from the use of this product.

Keyword Index (for Search Optimization)

Matrix Metalloproteinase 2, MMP‑2, MMP2, CLG4, CLG4A, MMP‑II, MONA, TBE‑1, PEX, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase

Note: The above information may change due to product optimization, upgrades, or other factors. For the most current details, please contact Yakein Co.