IFN-β ELISA KIT 干扰素βELISA试剂盒(小

产品概览

中文名称：IFN-β ELISA KIT 干扰素β ELISA试剂盒（小鼠）
英文名称：Mouse IFN-β ELISA Kit
货号：KTE3019-2
适用物种：小鼠（Mouse）
检测类型：ELISA（夹心法）
应用领域：细胞分析、蛋白定量（ELISA核心产品）

技术参数

检测范围	15.63 pg/mL – 1,000 pg/mL
灵敏度（检测下限）	15.63 pg/mL
样本类型	细胞培养上清、血清、血浆
主要检测指标	IFN-β（小鼠）
分子名称	IFN-β

试剂盒组成

• Mouse IFN-β Microplate
• Mouse IFN-β Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse IFN-β Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

核心优势

• 采用EliKine™双抗体夹心体系，具备高灵敏度和特异性。
• 对IFN-β近缘结构体的交叉反应水平低，背景噪声可控。
• 检测范围宽广，适用于低至中等浓度的样本定量。
• 标准品为冻干粉，可直接溶解使用，保证浓度准确。

实验原理简述

本套件基于经典的双抗体夹心ELISA：

1. 固相抗体预包被于96孔酶标板。
2. 加入样本或标准品，小鼠IFN-β与固相抗体特异结合。
3. 洗涤除去未结合组分后，加入生物素化检测抗体形成“捕获‑抗原‑检测”复合物。
4. 链霉亲和素‑HRP结合至生物素，随后加入TMB底物产生蓝色信号。
5. 终止液将颜色转为黄色，用酶标仪在450 nm读取OD值，构建标准曲线并计算样本浓度。

使用建议

• 推荐每个样本使用双孔或三孔复孔检测，以降低孔间变异。
• 使用一次性吸头避免交叉污染。
• 试剂使用前于室温平衡30 分钟，充分混匀（低速振荡或每10 分钟手动摇匀）。
• 未使用的酶标板请立即放回包装、密闭保存，4 ℃保存。

注意事项

• 不同批号或过期的试剂不可混用。
• 请严格按照瓶标说明进行储存。
• 操作过程中务必使用一次性耗材，防止交叉污染。

存储与运输

• 未开封试剂盒：避光保存于2 °C – 8 °C，保质期24个月。
• 启用后请参照说明书对各组分进行分装保存。
• 运输方式：冰袋（蓝冰）冷链运输。

法规声明

警告：本产品仅供科研使用，禁止用于人类或临床诊断。产品的推荐用途不构成对任何专利或许可证的侵权建议，使用本产品导致的专利纠纷或其他合规问题，供应商不承担责任。

产品包装 & 价格

规格	单价（CNY）	交货期
48 T	1,598.00	期货（5天内发货）
96 T	2,558.00	期货（5天内发货）

背景信息（参考）

干扰素（IFNs）是宿主细胞在面对病毒、细菌、寄生虫或肿瘤等病原体时分泌的信号蛋白。I型干扰素（包括IFN‑α、IFN‑β等）通过STAT1/STAT2通路调控多种免疫基因，发挥抗病毒、调节细胞增殖及免疫应答的功能。检测小鼠IFN‑β水平有助于解析病毒感染、免疫调节及药物干预等研究场景。本套件提供一种可靠的定量方法，适配常规酶标仪，实现高通量分析。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Chinese Name: IFN-β ELISA KIT Interferon β ELISA Kit (Mouse)
English Name: Mouse IFN-β ELISA Kit
Catalog No.: KTE3019-2
Applicable Species: Mouse
Assay Type: ELISA (sandwich)
Application Area: Cell analysis, protein quantification (core ELISA product)

Technical Specifications

Detection Range	15.63 pg/mL – 1,000 pg/mL
Sensitivity (Lower Limit of Detection)	15.63 pg/mL
Sample Types	Cell culture supernatant, serum, plasma
Primary Analyte	IFN-β (mouse)
Molecular Name	IFN-β

Kit Components

• Mouse IFN-β Microplate
• Mouse IFN-β Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse IFN-β Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Key Advantages

• Utilizes the EliKine™ dual‑antibody sandwich system, offering high sensitivity and specificity.
• Exhibits low cross‑reactivity with IFN‑β homologous structures, resulting in controllable background noise.
• Wide detection range, suitable for quantifying low to moderate concentrations.
• Standards are lyophilized powder, can be reconstituted directly to ensure accurate concentrations.

Brief Principle of the Assay

This kit is based on the classic dual‑antibody sandwich ELISA:

1. Capture antibody is pre‑coated on a 96‑well microtiter plate.
2. Sample or standard is added, and mouse IFN‑β specifically binds to the immobilized antibody.
3. After washing to remove unbound components, a biotinylated detection antibody is added to form a “capture‑antigen‑detection” complex.
4. Streptavidin‑HRP binds to biotin, and subsequent addition of TMB substrate generates a blue signal.
5. Stop solution converts the color to yellow; optical density is read at 450 nm with a plate reader, a standard curve is generated, and sample concentrations are calculated.

Recommended Usage

• It is recommended to run each sample in duplicate or triplicate wells to reduce inter‑well variability.
• Use disposable tips to avoid cross‑contamination.
• Allow reagents to equilibrate to room temperature for 30 minutes before use and mix thoroughly (gentle vortex or invert manually every 10 minutes).
• Unused microplates should be promptly returned to their packaging, sealed, and stored at 4 °C.

Precautions

• Do not mix reagents from different lot numbers or expired batches.
• Store strictly according to label instructions.
• Use disposable consumables throughout the procedure to prevent cross‑contamination.

Storage and Transportation

• Unopened kits: store protected from light at 2 °C – 8 °C; shelf life 24 months.
• After opening, aliquot and store individual components as described in the manual.
• Shipping: cold chain with ice packs (blue ice).

Regulatory Statement

Warning: This product is for research use only and must not be used in humans or for clinical diagnostics. The recommended use of the product does not constitute an infringement recommendation on any patent or license; the supplier assumes no liability for patent disputes or other compliance issues arising from use of this product.

Product Packaging & Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.00	Spot (dispatch within 5 days)
96 T	2,558.00	Spot (dispatch within 5 days)

Background Information (Reference)

Interferons (IFNs) are signaling proteins secreted by host cells in response to viruses, bacteria, parasites, or tumors. Type I interferons (including IFN‑α, IFN‑β, etc.) regulate numerous immune genes via the STAT1/STAT2 pathway, exerting antiviral, cell proliferation, and immune response functions. Measuring mouse IFN‑β levels facilitates the investigation of viral infection, immune modulation, and drug intervention studies. This kit provides a reliable quantitative method compatible with standard microplate readers for high‑throughput analysis.

Note: The above information may change due to product optimization or upgrades; please contact Yakogen for the most current details.