小鼠趋化因子20 ELISA定量试剂盒(CCL20/MIP-

产品概述

英文名称：Mouse CCL20/MIP-3α ELISA Kit
中文名称：小鼠趋化因子20 ELISA定量试剂盒（CCL20/MIP-3α）
货号：KTE3089-1
检测类型：酶联免疫吸附测定（ELISA）
反应物种：小鼠

核心应用

本套件专为 蛋白类 的 ELISA 检测设计，适用于细胞分析、免疫学及炎症信号通路研究，尤其是以下场景：

• 细胞培养上清、血清或血浆中 CCL20（MIP‑3α）的定量分析
• 炎症、免疫应答及黏膜免疫组织形成的机制研究
• 中枢神经系统自身免疫疾病模型中的趋化因子动态监测

检测原理概述

采用双抗体夹心法：

1. 微孔板预包被针对小鼠 CCL20 的固相抗体。
2. 样本或标准加入孔中，CCL20 与固相抗体形成捕获复合物。
3. 加入生物素化的检测抗体，形成双抗体桥。
4. 链霉亲和素‑HRP 结合到生物素标签，形成酶标记免疫复合物。
5. 加入 TMB 底物显色，终止后以 450 nm 测定 OD，OD 与样品中 CCL20 含量呈正相关。

关键特性 & 优势

• 高灵敏度：检测下限 62.5 pg/mL。
• 宽检测范围：62.5 pg/mL – 4,000 pg/mL。
• 特异性：针对 CCL20 的特异性抗体对同族分子无明显交叉。
• 采用 EliKine™ 技术，背景信号低，适合低丰度样本的定量。

适用样本类型

细胞培养上清、血清、血浆均可直接检测。建议使用预处理的无蛋白酶抑制剂的样本，以避免酶活性对结果的影响。

检测范围与灵敏度

检测范围	62.5 pg/mL – 4,000 pg/mL
灵敏度（LOD）	62.5 pg/mL

试剂盒组成

每套完整试剂盒包括：

• Mouse CCL20 Microplate
• Mouse CCL20 Standard (冻干粉)
• Standard Diluent
• Assay Buffer (10×)
• Mouse CCL20 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用要点（注意事项）

• 不同批号或过期的试剂不得混用。
• 使用前请在室温（≈25 °C）平衡 30 min。
• 实验操作建议使用一次性吸头，避免交叉污染。
• 未用完的板条请立即回包装、密封、在 4 °C 保存。
• 所有液体步骤需充分混匀，推荐使用低速振荡器或每 10 min 手动轻摇一次。
• 标准品与样品均建议做双孔或三孔复孔，以提高数据可靠性。

保存与运输

未开封	避光，2‑8 °C，保质期 24 个月。
开封后	请依据说明书对各组分进行分装保存。
运输条件	冰袋（蓝冰）冷链运输。

法规声明 & 警告

本产品仅限科研使用，不适用于人类或临床诊断。用户应确保在合规范围内使用本套件，任何因专利侵权或违规使用产生的后果，本公司概不负责。

产品规格 & 价格

规格	单价 (人民币)	交货期
48 T	1,598.0	请咨询
96 T	2,558.0	期货（5 天内发货）

订购方式

请直接联系商务代表或通过公司官网的 B2B 采购平台提交订单，注明货号 KTE3089-1 及所需规格。

背景信息

趋化因子配体 20 (CCL20)，亦称巨噬细胞炎性蛋白 3α (MIP‑3α)，属于 CC 趋化因子家族的小分子细胞因子。CCL20 能强烈趋化淋巴细胞、弱趋化中性粒细胞，广泛表达于单核细胞、巨噬细胞、神经胶质细胞、肠上皮细胞等多种组织。其通过与受体 CCR6 结合，介导黏膜淋巴组织的形成与功能，在中枢神经系统自身免疫疾病中也被认为具有重要作用。本套件提供了可靠的定量手段，帮助科研人员阐明 CCL20 在免疫调控中的具体角色。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse CCL20/MIP-3α ELISA Kit
Chinese Name: Mouse Chemokine Ligand 20 ELISA Quantitation Kit (CCL20/MIP-3α)
Catalog No.: KTE3089-1
Assay Type: Enzyme-Linked Immunosorbent Assay (ELISA)
Species: Mouse

Core Applications

This kit is specifically designed for protein ELISA detection and is suitable for cell analysis, immunology, and inflammation signaling pathway research, especially in the following scenarios:

• Quantitative analysis of CCL20 (MIP‑3α) in cell culture supernatants, serum, or plasma
• Mechanistic studies of inflammation, immune response, and mucosal immune tissue formation
• Dynamic monitoring of chemokines in central nervous system autoimmune disease models

Assay Principle Overview

Utilizes a double‑antibody sandwich format:

1. Microplate pre‑coated with solid‑phase capture antibody specific for mouse CCL20.
2. Sample or standard is added to the wells, allowing CCL20 to bind the capture antibody.
3. Biotinylated detection antibody is added, forming a sandwich complex.
4. Streptavidin‑HRP binds to the biotin tag, creating an enzyme‑labeled immune complex.
5. TMB substrate is added for color development; after stopping the reaction, OD is measured at 450 nm, which correlates positively with CCL20 concentration in the sample.

Key Features & Advantages

• High Sensitivity: Lower limit of detection 62.5 pg/mL.
• Wide Dynamic Range: 62.5 pg/mL – 4,000 pg/mL.
• Specificity: CCL20‑specific antibodies show no significant cross‑reactivity with homologous molecules.
• Employs EliKine™ technology, delivering low background signal and suitable for quantifying low‑abundance samples.

Compatible Sample Types

Cell culture supernatants, serum, and plasma can be assayed directly. It is recommended to use pretreated samples without protease inhibitors to avoid enzymatic activity affecting results.

Detection Range and Sensitivity

Detection Range	62.5 pg/mL – 4,000 pg/mL
Sensitivity (LOD)	62.5 pg/mL

Kit Components

Each complete kit includes:

• Mouse CCL20 Microplate
• Mouse CCL20 Standard (lyophilized powder)
• Standard Diluent
• Assay Buffer (10×)
• Mouse CCL20 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Critical Points (Precautions)

• Do not mix reagents from different lot numbers or expired reagents.
• Equilibrate at room temperature (≈25 °C) for 30 min prior to use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unutilized plate strips should be immediately resealed in their original packaging and stored at 4 °C.
• All liquid steps must be mixed thoroughly; low‑speed vortexing is recommended, or gently invert the plate every 10 min.
• Run standards and samples in duplicate or triplicate wells to enhance data reliability.

Storage & Shipping

Unopened	Protect from light, store at 2‑8 °C; shelf life 24 months.
After opening	Aliquot and store individual components as instructed in the manual.
Shipping Conditions	Cold‑chain shipping with insulated ice packs (blue ice).

Regulatory Statement & Warning

This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure compliance with applicable regulations; the company assumes no liability for consequences arising from patent infringement or unauthorized use.

Product Specifications & Pricing

Format	Unit Price (CNY)	Lead Time
48 T	1,598.0	Contact for details
96 T	2,558.0	Futures (shipment within 5 days)

Ordering

Please contact a sales representative directly or submit an order via the company's B2B procurement portal, quoting catalog number KTE3089-1 and the desired format.

Background Information

Chemokine (C‑C motif) ligand 20 (CCL20), also known as macrophage inflammatory protein‑3α (MIP‑3α), is a small cytokine belonging to the CC chemokine family. CCL20 potently chemoattracts lymphocytes and weakly attracts neutrophils, and is widely expressed in monocytes, macrophages, glial cells, intestinal epithelial cells, among other tissues. Through binding to its receptor CCR6, it mediates the formation and function of mucosal lymphoid tissues and is also considered to play a significant role in central nervous system autoimmune diseases. This kit offers a reliable quantitative method to assist researchers in elucidating the specific role of CCL20 in immune regulation.

Note: The information above may be subject to change due to product optimization or upgrades; please contact Yakeyin Corporation for the most current details.