小鼠白介素1受体拮抗剂 ELISA定量试剂盒(IL-1ra/

产品概述

货号：KTE3070-1 | 产品名称：小鼠白介素1受体拮抗剂 ELISA定量试剂盒 (IL-1ra/IL-1F3)

本产品为EliKine™系列核心产品，专为精准定量小鼠样本（细胞培养上清、血清、血浆）中的白介素1受体拮抗剂(IL-1ra/IL-1F3)而设计。采用成熟的夹心法ELISA原理，确保检测的高特异性和可靠性，适用于生命科学研究领域的细胞分析应用。

核心特性与优势

• 高灵敏度检测：最低检测浓度达31.25 pg/mL，能够精准捕获低丰度目标蛋白。
• 宽广的检测范围：31.25 pg/mL至2,000 pg/mL的动态范围，满足不同浓度样本的检测需求。
• 卓越的特异性：经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，结果可信度高。
• 即用型试剂盒：提供预包被微孔板、冻干标准品、检测抗体及全套优化缓冲液，简化实验流程。

试剂盒组分（完整列表）

• Mouse IL-1ra 预包被微孔板 (Mouse IL-1ra Microplate)
• Mouse IL-1ra 冻干标准品 (Mouse IL-1ra Standard, lyophilized)
• 10× 检测缓冲液 (Assay Buffer)
• 100× Mouse IL-1ra 检测抗体 (Mouse IL-1ra Detect Antibody)
• 100× 链霉亲和素-HRP (Streptavidin-HRP)
• HRP 底物 (TMB)
• 终止液 (Stop Solution)
• 20× 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

技术参数

产品类型	ELISA试剂盒	反应性	小鼠
检测方法	双抗体夹心法	样本类型	细胞培养上清、血清、血浆
检测范围	31.25 - 2,000 pg/mL	灵敏度	31.25 pg/mL
保存条件	未开封试剂盒避光保存于2-8℃，保质期24个月；启用后请参照说明书保存各组分。
运输条件	冰袋（蓝冰）运输，确保试剂活性。

产品规格与价格

规格	价格 (人民币)	货期
48 T	1,598.0	期货（5天内发货）
96 T	2,558.0	期货（5天内发货）

背景信息

白细胞介素-1受体拮抗剂(IL-1ra，亦称IL-1F3)是IL-1细胞因子家族的重要成员，能够特异性结合IL-1R1受体，有效抑制IL-1α和IL-1β的信号传导活性。小鼠IL-1ra是一种由多种细胞（如巨噬细胞、成纤维细胞、肝细胞等）分泌的22-25 kDa糖蛋白，在调控炎症反应、急性期蛋白产生及免疫细胞增殖等生理过程中发挥关键作用。本试剂盒基于双抗体夹心法原理，通过包被抗体捕获样本中的IL-1ra，再经生物素化检测抗体和链霉亲和素-HRP偶联物放大信号，最终通过TMB显色实现精准定量。

重要注意事项

• 请勿混合使用不同批号或过期试剂，避免交叉污染。
• 使用前所有试剂需室温平衡30分钟，并充分混匀。
• 建议对样品和标准品进行复孔（双孔或三孔）检测，以提高数据可靠性。
• 未使用的板条需立即密封并4℃保存。

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用者应确保应用符合相关法规和知识产权要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog Number: KTE3070-1 | Product Name: Mouse Interleukin-1 Receptor Antagonist ELISA Quantification Kit (IL-1ra/IL-1F3)

This product is a core offering of the EliKine™ series, specifically designed for the precise quantification of Mouse Interleukin-1 Receptor Antagonist (IL-1ra/IL-1F3) in mouse samples (cell culture supernatants, serum, plasma). Utilizing the established sandwich ELISA principle, it ensures high specificity and reliability, making it suitable for cellular analysis applications in life science research.

Core Features & Advantages

• High Sensitivity Detection: Minimum detectable concentration reaches 31.25 pg/mL, enabling accurate capture of low-abundance target proteins.
• Wide Detection Range: Dynamic range from 31.25 pg/mL to 2,000 pg/mL meets the detection requirements for samples with varying concentrations.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with analogues of the target protein, ensuring high result credibility.
• Ready-to-Use Kit: Provides pre-coated microplate, lyophilized standard, detection antibody, and a full set of optimized buffers to streamline experimental procedures.

Kit Components (Complete List)

• Mouse IL-1ra Pre-coated Microplate
• Mouse IL-1ra Standard, lyophilized
• 10× Assay Buffer
• 100× Mouse IL-1ra Detect Antibody
• 100× Streptavidin-HRP
• HRP Substrate (TMB)
• Stop Solution
• 20× Wash Buffer
• Plate Covers

Technical Parameters

Product Type	ELISA Kit	Reactivity	Mouse
Detection Method	Sandwich ELISA	Sample Type	Cell culture supernatants, Serum, Plasma
Detection Range	31.25 - 2,000 pg/mL	Sensitivity	31.25 pg/mL
Storage Conditions	Store unopened kit protected from light at 2-8°C; shelf life is 24 months. After opening, store components according to the manual.
Shipping Conditions	Shipped with ice packs (blue ice) to ensure reagent activity.

Specifications & Pricing

Specification	Price (RMB)	Availability
48 T	1,598.0	Future Supply (Ships within 5 days)
96 T	2,558.0	Future Supply (Ships within 5 days)

Background Information

Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a key member of the IL-1 cytokine family. It specifically binds to the IL-1R1 receptor, effectively inhibiting the signaling activity of IL-1α and IL-1β. Mouse IL-1ra is a 22-25 kDa glycoprotein secreted by various cells (such as macrophages, fibroblasts, hepatocytes, etc.) and plays a critical role in regulating inflammatory responses, acute-phase protein production, and immune cell proliferation. This kit is based on the sandwich ELISA principle: a coated antibody captures IL-1ra in the sample, followed by signal amplification using a biotinylated detection antibody and a streptavidin-HRP conjugate, with final quantification achieved via TMB colorimetric detection.

Important Notes

• Do not mix reagents from different lots or use expired reagents to avoid cross-contamination.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use and mix thoroughly.
• It is recommended to test samples and standards in replicates (duplicate or triplicate) to enhance data reliability.
• Unused strips should be sealed immediately and stored at 4°C.

Warning: This product is intended for research use only, not for human or clinical diagnostic use. Users should ensure their application complies with relevant regulations and intellectual property requirements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Abbkine for specific details.