细胞因子信号转导抑制因子1酶联免疫试剂盒(大鼠) JAB/C

产品概述

英文名称：Rat SOCS1 ELISA Kit (One Step Method)
中文名称：细胞因子信号转导抑制因子1酶联免疫试剂盒(大鼠) JAB/CIS1/SSI1/TIP3/CISH1/SSI-1/TIP-3/AISIMD/SOCS-1 ELISA KIT
货号：KTE4026-3
产品类别：核心产品 | 细胞分析 | ELISA

应用领域

• 蛋白类研究：用于大鼠SOCS1（抑制因子1）水平的定量分析。
• 一级应用：ELISA（酶联免疫吸附测定）
• 适用样本：血清、血浆、组织匀浆、细胞培养上清及其他相关液体样本。

关键特性 & 优势

• 高灵敏度：检测下限 0.05 ng/mL，检测范围 0.25‑20 ng/mL。
• 高特异性：采用双抗体夹心法，仅识别大鼠SOCS1，未发现明显交叉反应或背景干扰。
• 一步法简化操作：从加样到读数全程均可在 96 孔酶标板上完成，节约实验时间。
• EliKine™ 品牌优势：成熟的 ELISA 平台，结果可直接与已发布文献数据对比。

检测原理概述

本套件基于双抗体夹心法：

1. 预包被抗大鼠SOCS1 抗体的微孔板捕获样本中的 SOCS1。
2. 加入生物素化检测抗体，形成抗体‑抗原‑检测抗体复合物。
3. 链霉亲和素‑HRP 结合至检测抗体，完成酶标记。
4. 加入 TMB 底物（HRP Substrate A/B），在酶的催化下显色。
5. 加止液后变为黄色，用酶标仪在 450 nm 读取 OD 值，浓度与 OD 成正比。

试剂盒组成

• Rat SOCS1 Microplate（预包被板）
• Rat SOCS1 Standard (40 ng/mL)
• Standard Diluent
• Rat SOCS1 Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A / B（TMB 底物）
• Stop Solution
• Wash Buffer
• Plate Covers（封板盖）

操作要点 & 注意事项

• 所有试剂均须在使用前于室温（≈20‑25 °C）平衡 ≥30 分钟。
• 实验过程中推荐使用一次性吸头，防止交叉污染。
• 未使用的酶标板应立即放回原包装，密封后置于 4 °C 保存。
• 混匀过程对结果影响显著，建议使用低速振荡器或每 10 分钟手动摇匀一次。
• 样品和标准建议设置双孔或三孔复孔，以提高数据可靠性。
• 不同批号或超过有效期的试剂不应混用。

保存与运输

保存建议：未开封的试剂盒请避光保存在 2‑8 °C，保质期 6 个月。开启后请参照说明书对各组分分别保存。

运输条件：采用冰袋（蓝冰）冷链运输，确保全程低温。

规格 & 报价

警告 & 合规声明

本产品仅限**科研使用**，不适用于人类或临床诊断。请勿将本套件用于侵犯专利、许可证或其他法律规定的行为；如因违规使用产生的任何后果，供应商概不负责。

产品别名 & 检索关键词

JAB, CIS1, SSI1, TIP3, CISH1, SSI‑1, TIP‑3, AISIMD, SOCS‑1, JAK‑binding protein, STAT‑induced STAT inhibitor 1, Tec‑interacting protein 3 等。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Rat SOCS1 ELISA Kit (One Step Method)
Chinese Name: Cytokine signaling suppressor 1 Enzyme-Linked Immunosorbent Assay Kit (Rat) JAB/CIS1/SSI1/TIP3/CISH1/SSI-1/TIP-3/AISIMD/SOCS-1 ELISA KIT
Catalog Number: KTE4026-3
Product Category: Core Products | Cell Analysis | ELISA

Applications

• Protein Research: For quantitative analysis of Rat SOCS1 (Suppressor Of Cytokine Signaling 1) levels.
• Primary Application: ELISA (Enzyme-Linked Immunosorbent Assay)
• Suitable Samples: Serum, plasma, tissue homogenates, cell culture supernatants, and other related liquid samples.

Key Features & Advantages

• High Sensitivity: Lower detection limit 0.05 ng/mL, detection range 0.25‑20 ng/mL.
• High Specificity: Utilizes a sandwich ELISA method, specifically recognizes Rat SOCS1, no significant cross-reactivity or background interference observed.
• One-Step Simplified Procedure: The entire process from sample addition to reading can be completed on a 96-well microplate, saving experimental time.
• EliKine™ Brand Advantage: Mature ELISA platform, results are directly comparable with published literature data.

Detection Principle Overview

This kit is based on the sandwich ELISA principle:

1. Anti-Rat SOCS1 antibody pre-coated microplate captures SOCS1 in the sample.
2. Add biotinylated detection antibody to form an antibody‑antigen‑detection antibody complex.
3. Streptavidin‑HRP conjugates bind to the detection antibody, completing the enzyme labeling.
4. Add TMB substrate (HRP Substrate A/B), color development occurs catalyzed by the enzyme.
5. After adding stop solution, the color turns yellow. Measure the OD value at 450 nm using a microplate reader; concentration is proportional to the OD value.

Kit Components

• Rat SOCS1 Microplate (Pre-coated plate)
• Rat SOCS1 Standard (40 ng/mL)
• Standard Diluent
• Rat SOCS1 Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A / B (TMB Substrate)
• Stop Solution
• Wash Buffer
• Plate Covers

Key Procedures & Precautions

• All reagents must be equilibrated to room temperature (≈20‑25 °C) for ≥30 minutes before use.
• Use disposable pipette tips during the experiment to prevent cross-contamination.
• Unused microplate strips should be immediately returned to the original packaging, sealed, and stored at 4 °C.
• Mixing significantly impacts results; use a low-speed shaker or manually shake every 10 minutes.
• It is recommended to set up duplicate or triplicate wells for samples and standards to improve data reliability.
• Do not mix reagents from different lots or expired reagents.

Storage & Shipping

Storage Recommendation: Store the unopened kit protected from light at 2‑8 °C; shelf life is 6 months. After opening, refer to the instructions for storage conditions of individual components.

Shipping Conditions: Shipped with ice packs (blue ice) via cold chain to ensure low temperature throughout transit.

Specifications & Pricing

Warning & Compliance Statement

This product is intended for research use only, not for human or clinical diagnostic use. Do not use this kit in violation of patents, licenses, or other legal regulations; the supplier assumes no responsibility for any consequences arising from improper use.

Product Aliases & Search Keywords

JAB, CIS1, SSI1, TIP3, CISH1, SSI‑1, TIP‑3, AISIMD, SOCS‑1, JAK‑binding protein, STAT‑induced STAT inhibitor 1, Tec‑interacting protein 3, etc.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.