免疫球蛋白G酶联免疫试剂盒(大鼠) Immunoglobul

EliKine™ 大鼠免疫球蛋白G (IgG) ELISA试剂盒 (一步法)

货号: KTE4043-3

产品概述

EliKine™ 大鼠免疫球蛋白G (IgG) ELISA定量试剂盒是一款基于成熟双抗体夹心法原理的高性能检测工具，专门设计用于精准定量大鼠血清、血浆、组织匀浆及细胞培养上清液等样本中的IgG含量。本试剂盒作为细胞分析领域的核心产品，为生命科学研究提供可靠的数据支持。

核心特性与优势

• 高灵敏度与特异性: 最低检测限达0.15 μg/mL，确保对低丰度样本的准确检测。经过严格验证，与目标蛋白的类似物无明显交叉反应，有效降低背景干扰。
• 优化的检测范围: 宽达0.75 μg/mL至60 μg/mL的线性检测范围，满足绝大多数样本的检测需求，无需多次稀释，简化操作流程。
• 一步法操作: 实验流程经过优化，操作步骤精简，有效缩短整体检测时间，提升实验效率。
• 即用型组分: 试剂盒提供预包被板条、即用型标准品及缓冲液等完整组分，开盒即用，省去繁琐的准备工作。

检测原理简介

本试剂盒采用双抗体夹心酶联免疫吸附法（ELISA）。预包被于微孔板上的特异性抗体捕获样本中的大鼠IgG，随后依次与生物素化检测抗体及链霉亲和素-HRP结合，形成复合物。加入显色底物TMB后，在HRP催化下产生颜色反应，其强度与样本中的IgG浓度成正比。

试剂盒组分

• 预包被大鼠IgG微孔板 (Rat IgG Microplate)
• 大鼠IgG标准品 (120 μg/mL) (Rat IgG Standard)
• 标准品稀释液 (Standard Diluent)
• 大鼠IgG检测抗体 (Rat IgG Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A液与B液 (HRP Substrate A & B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

规格	价格 (人民币)	货期
48 T	1998.0	期货 (预计3个工作日内发货)
96 T	3198.0	期货 (预计3个工作日内发货)

保存与运输

保存建议: 未开封试剂盒请避光保存于2-8°C，保质期为6个月。启用后，请参照说明书分别保存各组分。

运输条件: 全程采用冰袋（蓝冰）低温运输，确保试剂活性。

重要注意事项

• 请勿将不同批次的试剂或过期试剂混合使用。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条需立即放回原包装袋并密封，置于4°C保存。
• 充分混匀试剂对结果准确性至关重要，建议使用低速振荡器或定期手动摇匀。
• 为保证结果可靠性，推荐对样品和标准品进行复孔（双孔或三孔）检测。

警告: 本产品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Rat Immunoglobulin G (IgG) ELISA Kit (One-Step)

Catalog Number: KTE4043-3

Product Overview

The EliKine™ Rat Immunoglobulin G (IgG) ELISA Quantitation Kit is a high-performance detection tool based on the established double-antibody sandwich principle, specifically designed for the accurate quantitation of IgG levels in samples such as rat serum, plasma, tissue homogenates, and cell culture supernatants. As a core product in cell analysis, this kit provides reliable data support for life science research.

Core Features & Advantages

• High Sensitivity & Specificity: Minimum detection limit as low as 0.15 μg/mL ensures accurate detection of low-abundance samples. Rigorously validated to show no significant cross-reactivity with analogs of the target protein, effectively reducing background interference.
• Optimized Detection Range: A broad linear detection range from 0.75 μg/mL to 60 μg/mL meets the detection needs of the vast majority of samples, eliminating the need for multiple dilutions and simplifying the workflow.
• One-Step Operation: The experimental procedure is optimized with streamlined steps, effectively reducing overall detection time and improving experimental efficiency.
• Ready-to-Use Components: The kit provides complete components including pre-coated strips, ready-to-use standards, and buffers, ready for immediate use, saving time on tedious preparations.

Detection Principle

This kit employs a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). A specific antibody pre-coated on the microplate captures rat IgG from the sample. This is followed by sequential binding with a biotinylated detection antibody and Streptavidin-HRP to form a complex. After adding the chromogenic substrate TMB, a color reaction catalyzed by HRP occurs, the intensity of which is proportional to the IgG concentration in the sample.

Kit Components

• Pre-coated Rat IgG Microplate
• Rat IgG Standard (120 μg/mL)
• Standard Diluent
• Rat IgG Detect Antibody
• Streptavidin-HRP
• HRP Substrate A & B
• Stop Solution
• Wash Buffer
• Plate Covers

Specifications & Pricing

Specification	Price (RMB)	Lead Time
48 T	1998.0	Future Supply (Estimated shipment within 3 business days)
96 T	3198.0	Future Supply (Estimated shipment within 3 business days)

Storage & Shipping

Storage Recommendation: Store the unopened kit protected from light at 2-8°C; shelf life is 6 months. After opening, please store components separately according to the instructions.

Shipping Conditions: Shipped with ice packs (blue ice) to maintain low temperature, ensuring reagent activity.

Important Notes

• Do not mix reagents from different batches or use expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, always use disposable pipette tips during the experiment.
• Unused strips should be immediately returned to the original pouch and sealed, stored at 4°C.
• Thorough mixing of reagents is crucial for accurate results; use a low-speed shaker or periodic manual shaking is recommended.
• For result reliability, it is recommended to test samples and standards in replicates (duplicate or triplicate wells).

Warning: This product is for research use only, not intended for human or clinical diagnostic use. Users should ensure their application does not infringe relevant patents or license agreements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.