大鼠去甲肾`上`腺`素 ELISA定量试剂盒(NE/NA/N

大鼠去甲肾`上`腺`素（NE）ELISA 定量试剂盒 (One‑Step Method)

产品型号：KTE4030-1  |  英文名称：Rat Noradrenaline (NE) ELISA Kit (One Step Method)

产品概述

本试剂盒基于 EliKine™ 双抗体夹心 ELISA 技术，专为 大鼠 去甲肾`上`腺`素（Noradrenaline, NE/NA/NAd/Norepi）在血清、血浆、组织匀浆、细胞上清及其他液体样本中的定量检测设计。

核心应用

• 蛋白类分析，尤其是神经递质及应激相关研究
• ELISA 体系的基础与高级实验（单孔/双孔/三孔复孔检测）
• 细胞分析中心的常规检测平台

检测原理

采用 双抗体夹心法：酶标板预包被特异性抗大鼠去甲肾`上`腺`素抗体，样品或标准加入后形成抗原–抗体复合物，随后加入生物素化检测抗体和链霉亲和素‑HRP，完成抗体‑抗原‑抗体‑酶的复合结构。洗脱后加入 TMB 底物，在 HRP 催化下显色（蓝 → 黄），使用 450 nm 波长读取 OD 值，OD 与 NE 浓度呈正比。

试剂盒组成

• Rat NE Microplate（预包被酶标板）
• Rat NE Standard（浓度 1,600 pg/mL）
• Standard Diluent（稀释缓冲液）
• Rat NE Detect Antibody（检测抗体）
• Streptavidin‑HRP
• HRP Substrate A & B（TMB 底物）
• Stop Solution（终止液）
• Wash Buffer
• Plate Covers（防尘盖）

性能指标

检测范围	10 pg/mL – 800 pg/mL
灵敏度（LOD）	2 pg/mL
特异性	未检测到显著交叉反应或背景干扰

样本要求

适用于以下样本类型：

• 血清 / 血浆
• 组织匀浆（如脑、肾上腺）
• 细胞培养上清
• 其他相关液体样本

使用提示

• 试剂请严格按照瓶贴说明保存，使用前在室温放置 30 分钟平衡。
• 建议使用一次性吸头以防交叉污染。
• 未使用的酶标板应立即放回包装，密封后置于 4 ℃保存。
• 混匀对结果影响显著，推荐低速振荡或每 10 分钟手动轻摇。
• 标准品与样品建议做双孔或三孔复孔，提高数据可靠性。

储存与运输

• 未开封试剂盒：避光保存于 2‑8 ℃，保质期 6 个月。
• 开封后各组分请参考说明书中具体储存条件。
• 运输方式：冷链（蓝冰）包装，确保全程低温。

订购规格 & 价格

规格	单价（人民币）	交货期限
48 T	1,998.00	期货（3 天内发货）
96 T	3,198.00	期货（3 天内发货）

合规声明

仅限科研使用。本产品不适用于人类临床诊断或任何形式的治疗。使用本试剂盒进行的实验不应违反任何专利、许可证或法规，因专利侵权或其他违规所产生的后果，本公司概不负责。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat Noradrenaline (NE) ELISA Quantification Kit (One‑Step Method)

Product Model: KTE4030-1  |  English Name: Rat Noradrenaline (NE) ELISA Kit (One Step Method)

Product Overview

This kit is based on EliKine™ double‑antibody sandwich ELISA technology and is designed for quantitative detection of rat noradrenaline (Noradrenaline, NE/NA/NAd/Norepi) in serum, plasma, tissue homogenates, cell supernatants, and other liquid samples.

Core Applications

• Protein analysis, particularly neurotransmitter and stress‑related studies
• Fundamental and advanced ELISA assays (single‑well/duplicate/triplicate multiplex detection)
• Routine testing platform for cellular analysis cores

Assay Principle

Utilizes a double‑antibody sandwich format: the microplate is pre‑coated with a specific anti‑rat noradrenaline capture antibody. Upon addition of sample or standard, an antigen–antibody complex forms, followed by addition of a biotinylated detection antibody and streptavidin‑HRP, generating a capture‑antigen‑detection‑enzyme complex. After washing, TMB substrate is added; HRP catalyzes a color change (blue → yellow). Optical density is measured at 450 nm, and the OD is directly proportional to NE concentration.

Kit Components

• Rat NE Microplate (pre‑coated plate)
• Rat NE Standard (1,600 pg/mL)
• Standard Diluent (dilution buffer)
• Rat NE Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB substrate)
• Stop Solution
• Wash Buffer
• Plate Covers (dust‑proof lids)

Performance Specifications

Detection Range	10 pg/mL – 800 pg/mL
Sensitivity (LOD)	2 pg/mL
Specificity	No significant cross‑reactivity or background interference detected

Sample Requirements

Suitable for the following sample types:

• Serum / Plasma
• Tissue homogenates (e.g., brain, adrenal gland)
• Cell culture supernatants
• Other relevant liquid samples

Usage Tips

• Store reagents strictly according to label instructions; equilibrate at room temperature for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unused microplates should be promptly returned to their packaging, sealed, and stored at 4 °C.
• Mixing critically affects results; recommend low‑speed vortexing or gentle manual inversion every 10 minutes.
• Run standards and samples in duplicate or triplicate to enhance data reliability.

Storage and Shipping

• Unopened kits: store protected from light at 2‑8 °C; shelf life 6 months.
• After opening, refer to the instruction manual for specific storage conditions of each component.
• Shipping: cold‑chain (dry ice) packaging to ensure continuous low temperature.

Ordering Specifications & Price

Specification	Unit Price (CNY)	Delivery Lead Time
48 T	1,998.00	Spot (shipment within 3 days)
96 T	3,198.00	Spot (shipment within 3 days)

Regulatory Statement

For research use only. This product is not intended for human clinical diagnosis or any form of therapy. Experiments performed with this kit must not infringe any patents, licenses, or regulations; the company assumes no responsibility for consequences arising from patent infringement or other violations.

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakein Co.