趋化因子2酶联免疫试剂盒(大鼠) CXCL2/CINC-3

EliKine™ 大鼠趋化因子2 (CXCL2/CINC-3) ELISA试剂盒 - 高灵敏度定量检测方案

产品概述

货号: KTE3021-3
产品名称: 趋化因子2酶联免疫试剂盒(大鼠) CXCL2/CINC-3 ELISA KIT
产品类型: ELISA核心产品 - 夹心法检测
反应性: 大鼠
样本类型: 细胞培养上清、血清、血浆

核心特性与优势

• 高灵敏度检测: 最低检测浓度达31.25 pg/mL，确保低丰度蛋白的准确检测
• 优异特异性: EliKine™技术平台有效避免目标蛋白类似物的交叉反应，显著降低背景干扰
• 宽线性范围: 31.25-2,000 pg/mL的检测范围满足不同浓度样本的检测需求
• 稳定可靠的性能: 优化的试剂配方确保实验结果的可重复性和准确性

技术参数

参数类别	详细规格
检测方法	双抗体夹心ELISA法
检测范围	31.25 pg/mL - 2,000 pg/mL
灵敏度	31.25 pg/mL
孵育时间	约3.5小时（含标准品制备时间）
样本体积	每孔100μL（推荐稀释后体积）

试剂盒组分

• 大鼠CXCL2/CINC-3预包被微孔板
• 大鼠CXCL2/CINC-3标准品（冻干粉）
• 标准品稀释液
• 检测缓冲液(10×)
• 大鼠CXCL2/CINC-3检测抗体(100×)
• 链霉亲和素-HRP偶联物(100×)
• HRP底物（TMB）
• 终止液
• 洗涤缓冲液(20×)
• 板盖

产品规格与定价

规格	价格（元）	货期
48 T	1,598.0	请咨询
96 T	2,558.0	期货（5天内发货）

背景信息

趋化因子CXC配体2 (CXCL2)是CXC趋化因子家族中的重要成员，也称为巨噬细胞炎症蛋白2 (MIP-2)或生长调节致癌基因β (Gro-β)。该细胞因子主要由单核细胞和巨噬细胞分泌，在炎症反应和免疫调节中发挥关键作用，特别对中性粒细胞和造血干细胞具有显著的趋化活性。

实验注意事项

• 请勿混合使用不同批号的试剂或过期试剂
• 使用前所有试剂需室温平衡30分钟
• 实验过程中请使用一次性吸头，避免交叉污染
• 推荐使用低速频率振荡器或每10分钟手动混匀
• 建议样品和标准品进行双孔或三孔检测（复孔）

保存与运输

保存条件: 未开封试剂盒避光保存于2-8℃，保质期24个月
运输条件: 冰袋运输（蓝冰）

重要声明

本产品仅供研究使用，不适用于人类或临床诊断。使用前请确认符合当地法规要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Rat Chemokine CXCL2 (CINC-3) ELISA Kit - High Sensitivity Quantitative Assay

Product Overview

Catalog No.: KTE3021-3
Product Name: Chemokine CXCL2 ELISA Kit (Rat) CXCL2/CINC-3 ELISA KIT
Product Type: ELISA Core Product – Sandwich Format
Species Reactivity: Rat
Sample Types: Cell culture supernatants, serum, plasma

Key Features and Advantages

• High Sensitivity: Lower limit of detection (LLD) of 31.25 pg/mL, ensuring accurate quantification of low‑abundance proteins
• Excellent Specificity: EliKine™ technology minimizes cross‑reactivity with homologous proteins, markedly reducing background interference
• Broad Linear Range: 31.25–2,000 pg/mL accommodates samples with varying concentrations
• Stable and Reliable Performance: Optimized reagent formulation ensures reproducibility and accuracy

Technical Specifications

Parameter	Specification
Assay Method	Dual‑antibody sandwich ELISA
Detection Range	31.25 pg/mL – 2,000 pg/mL
Sensitivity	31.25 pg/mL
Incubation Time	Approximately 3.5 h (including standard preparation)
Sample Volume	100 µL per well (recommended diluted volume)

Kit Components

• Rat CXCL2/CINC-3 Pre‑coated Microplate
• Rat CXCL2/CINC-3 Standard (lyophilized powder)
• Standard Diluent
• Assay Buffer (10×)
• Rat CXCL2/CINC-3 Detection Antibody (100×)
• Streptavidin‑HRP Conjugate (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Seal

Product Formats and Pricing

Format	Price (CNY)	Lead Time
48‑well (48 T)	1,598.0	Please inquire
96‑well (96 T)	2,558.0	Stock (shipping within 5 days)

Background

Chemokine (C‑X‑C motif) ligand 2 (CXCL2) is a prominent member of the CXC chemokine family, also known as macrophage inflammatory protein‑2 (MIP‑2) or growth‑regulated oncogene β (Gro‑β). This cytokine is primarily secreted by monocytes and macrophages and plays a pivotal role in inflammatory responses and immune regulation, exhibiting potent chemotactic activity for neutrophils and hematopoietic stem cells.

Experimental Considerations

• Do not mix reagents from different batches or use expired reagents
• Allow all reagents to equilibrate to room temperature for 30 min before use
• Use disposable pipette tips throughout the procedure to avoid cross‑contamination
• Recommend gentle agitation on a low‑speed orbital shaker or manual mixing every 10 min
• Perform samples and standards in duplicate or triplicate wells (replicates)

Storage and Shipping

Storage Conditions: Store unopened kits protected from light at 2–8 °C. Shelf life: 24 months
Shipping Conditions: Ship on ice packs (blue ice)

Important Statement

This product is for research use only and is not intended for human or clinical diagnostic applications. Please verify compliance with local regulations prior to use.

Note: The information above is subject to change due to product optimization or upgrades. For the most current details, please contact EliKine™.