IgG1/IGHG1 ELISA KIT 免疫球蛋白G1EL

产品概览

中文名称：IgG1/IGHG1 ELISA KIT 免疫球蛋白G1 ELISA试剂盒（大鼠）
英文名称：Rat IgG1 ELISA Kit (One Step Method)
货号：KTE4045-2
产品分类：核心产品 → 细胞分析 → ELISA

适用领域

• 产品应用：蛋白类
• 一级应用：ELISA应用
• 检测类型：ELISA（双抗体夹心法）
• 样本类型：血清、血浆、组织、细胞上清及相关液体样本

技术原理

本套装采用双抗体夹心法，通过特异性抗体捕获大鼠免疫球蛋白 G1（IgG1），随后加入生物素标记检测抗体与链霉亲和素‑HRP形成完整的抗体‑抗原‑抗体‑酶复合物。经洗涤后加入TMB显色底物，在HRP催化下产生蓝色反应，终止液将颜色转为黄色。使用酶标仪在 450 nm 测定光密度（OD），OD 与样本中 IgG1 浓度呈正比，实现定量分析。

关键性能

• 检测范围：0.5 μg/mL ~ 40 μg/mL
• 灵敏度：0.1 μg/mL
• 特异性：针对大鼠 IgG1，无显著交叉反应或背景干扰

试剂盒组成

• Rat IgG1 Complex Microplate
• Rat IgG1 Complex Standard (80 µg/mL)
• Standard Diluent
• Rat IgG1 Complex Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B
• Stop Solution
• Wash Buffer
• Plate Covers

使用指南

实验前准备

• 试剂须在使用前于室温（约 20‑25 °C）平衡 30 分钟。
• 全部操作使用一次性吸头，防止交叉污染。
• 未使用的酶标板需立即放回包装，密封后冷藏（4 °C）保存。
• 混匀步骤建议使用低速振荡或每 10 分钟手动轻摇一次，以确保均匀反应。

检测流程概述（一步法）

1. 向已包被抗体的微孔板中加入样本或标准品。
2. 加入 Rat IgG1 Complex Detect Antibody，形成第一层抗体‑抗原复合物。
3. 加入 Streptavidin‑HRP，完成抗体‑抗原‑检测抗体‑酶的四元结构。
4. 洗涤除去未结合物。
5. 依次加入 HRP Substrate A 与 B，显色后加入 Stop Solution。
6. 使用酶标仪在 450 nm 读取 OD 值，计算样本 IgG1 浓度。

实验建议

• 样品及标准品建议设置双孔或三孔复孔，以提升数据可靠性。
• 若样本稀释度较大，建议先进行预稀释后再加入微孔板。

储存与运输

• 未开封保存：避光，2‑8 °C，保质期 6 个月。
• 开封后：请参照说明书对各组分分别储存。
• 运输条件：冰袋（蓝冰）冷藏运输，确保全程温度控制。

规格与订购信息

规格	套数/孔数	单价 (¥)	货期
规格 1	48 T	1998.00	期货（3 天内发货）
规格 2	96 T	3198.00	期货（3 天内发货）

合规与免责声明

本产品仅供科研使用，不得用于人类临床诊断。使用本套装进行的实验应遵守所在地区的法规与专利要求。因使用本产品可能产生的专利侵权或其他违规行为，供应商不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Chinese Name: IgG1/IGHG1 ELISA KIT Immunoglobulin G1 ELISA Reagent Kit (Rat)
English Name: Rat IgG1 ELISA Kit (One Step Method)
Catalog No.: KTE4045-2
Product Category: Core Products → Cell Analysis → ELISA

Applicable Fields

• Product Application: Protein
• Primary Application: ELISA
• Assay Type: ELISA (sandwich double‑antibody method)
• Sample Types: Serum, plasma, tissue, cell supernatant and related liquid samples

Technical Principle

This kit employs a sandwich double‑antibody format. Specific capture antibodies bind rat immunoglobulin G1 (IgG1). Subsequently, a biotin‑labeled detection antibody and streptavidin‑HRP form a complete antibody‑antigen‑antibody‑enzyme complex. After washing, TMB substrate is added; HRP catalyzes a blue reaction, which is stopped to yield a yellow color. Optical density (OD) is measured at 450 nm using a plate reader. OD is directly proportional to the IgG1 concentration in the sample, enabling quantitative analysis.

Key Performance

• Detection Range: 0.5 µg/mL ~ 40 µg/mL
• Sensitivity: 0.1 µg/mL
• Specificity: Specific for rat IgG1 with no significant cross‑reactivity or background interference

Kit Components

• Rat IgG1 Complex Microplate
• Rat IgG1 Complex Standard (80 µg/mL)
• Standard Diluent
• Rat IgG1 Complex Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B
• Stop Solution
• Wash Buffer
• Plate Covers

Usage Guide

Pre‑Experiment Preparation

• Reagents should be equilibrated at room temperature (≈ 20‑25 °C) for 30 minutes before use.
• Use disposable tips for all steps to prevent cross‑contamination.
• Unopened microplates must be returned to their original packaging, sealed, and stored refrigerated (4 °C).
• Mixing is recommended by low‑speed vortexing or gentle inversion every 10 minutes to ensure uniform reactions.

Assay Workflow Overview (One‑Step Method)

1. Add sample or standard to the antibody‑coated microplate wells.
2. Add Rat IgG1 Complex Detect Antibody to form the first antibody‑antigen complex.
3. Add Streptavidin‑HRP to complete the four‑component antibody‑antigen‑detect antibody‑enzyme structure.
4. Wash to remove unbound material.
5. Sequentially add HRP Substrate A and B; after color development, add Stop Solution.
6. Read OD at 450 nm with a plate reader and calculate the IgG1 concentration of the samples.

Experimental Recommendations

• Run samples and standards in duplicate or triplicate wells to improve data reliability.
• If samples require high dilution, perform a pre‑dilution before adding to the microplate.

Storage and Shipping

• Unopened Storage: Protect from light, 2‑8 °C, shelf life 6 months.
• After Opening: Follow the instruction manual for individual component storage.
• Shipping Conditions: Cold‑chain transport with blue ice packs, ensuring temperature control throughout.

Specifications and Ordering Information

Specification	Sets/Well Count	Unit Price (¥)	Lead Time
Spec 1	48 T	1998.00	In‑stock (ship within 3 days)
Spec 2	96 T	3198.00	In‑stock (ship within 3 days)

Regulatory and Disclaimer

This product is intended for research use only and must not be used for human clinical diagnostics. Experiments performed with this kit should comply with local regulations and patent requirements. The supplier assumes no liability for any patent infringement or other violations arising from the use of this product.

Note: The information above may change due to product optimization or upgrades; please contact Yakein Co., Ltd. for the most current details.