CIS3/SSI3/ATOD4/Cish3/SSI-3/SO

Rat SOCS3 ELISA Kit (One Step Method) – 大鼠细胞因子信号转导抑制因子 3 ELISA 试剂盒

产品概览

货号：KTE4027-2   规格：48 T / 96 T   适用种属：大鼠

本套装为EliKine™核心产品，专为细胞分析中的ELISA应用设计，采用双抗体夹心法，实现对大鼠SOCS3蛋白的高灵敏、特异定量检测。

关键参数

试剂盒组成

• Rat SOCS3 Microplate（预包被抗体孔板）
• Rat SOCS3 Standard（1,600 pg/mL）
• Standard Diluent
• Rat SOCS3 Detect Antibody（生物素标记）
• Streptavidin‑HRP
• HRP Substrate A & B（TMB显色底物）
• Stop Solution
• Wash Buffer
• Plate Covers

技术要点

1. **双抗体夹心原理**：孔板表面预包被特异性捕获抗体，加入样本后SOCS3分子被捕获；随后加入生物素化检测抗体形成免疫复合物，再通过链霉亲和素‑HRP实现酶标记。

2. **一步法操作**：本套装采用一次加样、一次显色的“One Step”流程，降低操作步骤，提升实验一致性。

3. **高特异性**：经验证对SOCS3同源蛋白（如CIS、SSI‑3等）不存在显著交叉反应，背景信号低。

实验流程概览

1. 将样本或标准品加入预包被孔板，37 ℃孵育1 h。
2. 洗板后加入生物素化检测抗体，室温孵育30 min。
3. 加入Streptavidin‑HRP，继续孵育15 min。
4. 使用TMB底物显色至蓝色，随后加Stop Solution终止反应（变为黄色）。
5. 在450 nm波长读取OD值，绘制标准曲线并计算样本中SOCS3浓度。

使用建议

• 使用前请在室温（约20‑25 ℃）平衡30 分钟。
• 全程使用一次性吸头，避免交叉污染。
• 未使用的微孔板请立即放回包装中，密闭保存于4 ℃。
• 建议每个样本或标准品做双孔或三孔复孔，以提高结果可靠性。
• 充分混匀（低速振荡或每10 分钟手动摇匀）对实验结果尤为关键。

适用场景

本试剂盒适用于科研机构、制药企业及生物技术公司在以下领域的定量分析：

• 炎症反应与细胞因子信号通路研究
• STAT‑SOCS负反馈机制的功能验证
• 动物模型（大鼠）药理学及毒理学评估
• 基因敲除或过表达模型中SOCS3表达水平的监测

重要声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套装不构成对任何专利或许可证的侵权行为的建议，若因使用产生的专利纠纷或其他违规行为，供应商不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat SOCS3 ELISA Kit (One-Step Method) – Rat Cytokine Signal Transducer Inhibitor 3 ELISA Kit

Product Overview

Catalog No.: KTE4027-2   Specification: 48 T / 96 T   Applicable Species: Rat

This kit is an EliKine™ core product, specially designed for ELISA applications in cellular analysis, employing a sandwich ELISA format to achieve highly sensitive and specific quantitative detection of rat SOCS3 protein.

Key Specifications

Kit Components

• Rat SOCS3 Microplate (pre‑coated antibody plate)
• Rat SOCS3 Standard (1,600 pg/mL)
• Standard Diluent
• Rat SOCS3 Detection Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A & B (TMB chromogenic substrate)
• Stop Solution
• Wash Buffer
• Plate Covers

Technical Highlights

1. **Sandwich ELISA Principle**: The plate surface is pre‑coated with a specific capture antibody; upon addition of the sample, SOCS3 molecules are captured. Subsequently, a biotinylated detection antibody is added to form an immune complex, followed by Streptavidin‑HRP for enzymatic labeling.

2. **One-Step Procedure**: This kit utilizes a single addition and single color development “One Step” workflow, reducing procedural steps and enhancing assay consistency.

3. **High Specificity**: Validated to show no significant cross‑reactivity with SOCS3 homologues (e.g., CIS, SSI‑3), resulting in low background signal.

Assay Workflow Overview

1. Add sample or standard to the pre‑coated plate and incubate at 37 °C for 1 hour.
2. After washing, add biotinylated detection antibody and incubate at room temperature for 30 minutes.
3. Add Streptavidin‑HRP and incubate for an additional 15 minutes.
4. Develop color with TMB substrate until a blue color appears, then add Stop Solution to terminate the reaction (turns yellow).
5. Read absorbance at 450 nm, construct a standard curve, and calculate SOCS3 concentration in the samples.

Recommended Usage

• Allow the kit to equilibrate at room temperature (≈20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout to avoid cross‑contamination.
• Unutilized microplate should be promptly returned to its packaging and stored sealed at 4 °C.
• It is recommended to run each sample or standard in duplicate or triplicate wells to improve result reliability.
• Thorough mixing (gentle vortex or manual inversion every 10 minutes) is critical for assay performance.

Applicable Scenarios

This kit is suitable for quantitative analyses in the following fields for academic institutions, pharmaceutical companies, and biotechnology firms:

• Inflammatory response and cytokine signaling pathway studies
• Functional validation of STAT‑SOCS negative feedback mechanisms
• Pharmacology and toxicology assessment in animal (rat) models
• Monitoring SOCS3 expression levels in gene knockout or overexpression models

Important Disclaimer

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Use of this kit does not constitute advice regarding infringement of any patents or licenses; the supplier shall not be liable for any patent disputes or other violations arising from its use.

Note: The above information may be subject to change due to product optimization or upgrades; please contact Yake‑In for the most current details.