大鼠骨桥蛋白 ELISA定量试剂盒(Osteopontin/

大鼠骨桥蛋白(OPN/SPP1) ELISA定量试剂盒 (一步法)

货号: KTE4077-1

产品分类: 核心产品 - 细胞分析

产品概述

本产品是一款专为大鼠样本设计的高灵敏度ELISA检测试剂盒，采用成熟的双抗体夹心一步法原理，用于精准定量大鼠血清、血浆、组织匀浆及细胞培养上清等样本中的骨桥蛋白(OPN/SPP1)含量。该试剂盒属于EliKine™系列，旨在为骨代谢、免疫反应及肿瘤微环境等基础研究与药物开发领域提供可靠的分析工具。

核心特性与优势

• 高灵敏度与特异性: 最低检测浓度达10 pg/mL，线性范围覆盖50 pg/mL - 4000 pg/mL。经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰。
• 一步法操作便捷: 优化后的实验流程减少了操作步骤，有助于提升实验效率并降低误差。
• 广泛的样本兼容性: 适用于血清、血浆、组织匀浆、细胞上清液等多种生物液体样本。
• 批次稳定性: 严格的质量控制确保不同批次间结果的一致性。

检测原理简介

本试剂盒采用双抗体夹心ELISA技术。预包被于微孔板的大鼠OPN特异性捕获抗体与样本中的OPN结合后，依次加入生物素标记的检测抗体及HRP标记的链霉亲和素，形成复合物。加入TMB底物显色，颜色深度与样本中OPN浓度成正比。通过450 nm波长下测定OD值，并参照标准曲线即可实现精准定量。

试剂盒组分

• 预包被大鼠OPN/SPP1微孔板
• 大鼠OPN/SPP1标准品 (8000 pg/mL)
• 标准品稀释液
• 生物素标记的大鼠OPN/SPP1检测抗体
• HRP酶标记试剂
• 显色液A & 显色液B
• 终止液
• 洗涤缓冲液
• 板盖

技术参数

检测范围	50 - 4000 pg/mL
灵敏度	10 pg/mL
样本类型	血清、血浆、组织匀浆、细胞上清液等
反应性	大鼠
检测方法	双抗体夹心一步法ELISA
孵育时间	约 2.5 小时（具体步骤参考说明书）

订购信息

规格	价格 (人民币)	货期
48 T	1,998	期货（3个工作日内发货）
96 T	3,198	期货（3个工作日内发货）

保存与运输

保存建议: 未开封试剂盒请避光保存于2-8°C，有效期6个月。启用后请按说明书要求分别保存各组分。

运输条件: 全程采用蓝冰冰袋低温运输，以确保试剂活性。

重要提示

• 请严格按照说明书进行操作，不同批号试剂请勿混用。
• 使用前请将所有试剂组分室温平衡约30分钟。
• 为避免交叉污染，建议实验全程使用一次性吸头。
• 为确保实验准确性，建议对标准品和样本进行复孔检测（双孔或三孔）。
• 本产品仅供科研使用，不适用于临床诊断。

注：骨桥蛋白(OPN)也称为SPP1、BNSP、BSPI等，是一种参与细胞粘附、迁移及免疫调节等多种生理病理过程的重要细胞外基质蛋白。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat Osteopontin (OPN/SPP1) ELISA Quantitation Kit (One‑step)

Part Number: KTE4077-1

Product Category: Core Product – Cell Analysis

Product Overview

This product is a high‑sensitivity ELISA detection kit specifically designed for rat samples. It employs a validated double‑antibody sandwich one‑step format for precise quantification of osteopontin (OPN/SPP1) in rat serum, plasma, tissue homogenates, and cell‑culture supernatants. The kit belongs to the EliKine™ series and is intended to provide a reliable analytical tool for basic research and drug development in bone metabolism, immune response, and tumor microenvironment studies.

Key Features and Advantages

• High Sensitivity and Specificity: Minimum detectable concentration of 10 pg/mL; linear range spans 50 pg/mL – 4000 pg/mL. Rigorous validation demonstrates negligible cross‑reactivity or background interference with homologous proteins.
• One‑step Convenience: Optimized workflow reduces procedural steps, enhancing throughput and minimizing error.
• Broad Sample Compatibility: Suitable for serum, plasma, tissue homogenates, cell supernatants, and other biological fluids.
• Batch‑to‑Batch Consistency: Strict quality control ensures uniform performance across production lots.

Assay Principle Summary

The kit utilizes double‑antibody sandwich ELISA technology. Pre‑coated rat OPN‑specific capture antibodies on the microplate bind OPN present in the sample, followed by addition of biotinylated detection antibody and HRP‑conjugated streptavidin to form a complex. After adding TMB substrate, color development is proportional to OPN concentration. Optical density is measured at 450 nm and quantified against a standard curve.

Kit Components

• Pre‑coated rat OPN/SPP1 microplate
• Rat OPN/SPP1 standard (8000 pg/mL)
• Standard diluent
• Biotin‑labeled rat OPN/SPP1 detection antibody
• HRP conjugate
• Substrate Solution A & Substrate Solution B
• Stop solution
• Wash buffer
• Plate seal

Technical Specifications

Detection Range	50 – 4000 pg/mL
Sensitivity	10 pg/mL
Sample Types	Serum, plasma, tissue homogenate, cell supernatant, etc.
Species Reactivity	Rat
Assay Method	Double‑antibody sandwich one‑step ELISA
Incubation Time	Approximately 2.5 h (see protocol for detailed steps)

Ordering Information

Format	Price (CNY)	Lead Time
48 T	1,998	Spot shipment (dispatch within 3 business days)
96 T	3,198	Spot shipment (dispatch within 3 business days)

Storage and Transportation

Storage Recommendation: Store unopened kits protected from light at 2–8 °C; shelf life is 6 months. After opening, store each component according to the instructions.

Shipping Conditions: Ship with cold‑pack gel packs to maintain low temperature and preserve reagent activity.

Important Notices

• Follow the protocol precisely; do not mix reagents from different lot numbers.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross‑contamination.
• For optimal accuracy, run standards and samples in duplicate or triplicate (duplicate or triplicate wells).
• This product is intended for research use only and is not suitable for clinical diagnostics.

Note: Osteopontin (OPN), also referred to as SPP1, BNSP, BSPI, etc., is an extracellular matrix protein that plays key roles in cell adhesion, migration, and immune regulation across various physiological and pathological processes.

Note: Information is subject to change due to product upgrades or other factors; please contact Yakei Inc. for the most current details.