表皮生长因子酶联免疫试剂盒(大鼠) EGF ELISA KI

Rat EGF ELISA Kit (KTE3074-3)

产品概览

中文名称：表皮生长因子酶联免疫试剂盒（大鼠）
英文名称：Rat EGF ELISA Kit
货号：KTE3074-3
适用物种：大鼠
检测类型：ELISA（夹心法）
样本来源：细胞培养上清、血清、血浆

核心技术与优势

• 基于EliKine™ 双抗体夹心法，提供极高的灵敏度（15.63 pg/mL）和特异性。
• 检测范围覆盖 15.63 pg/mL – 1,000 pg/mL，满足低至中等浓度的定量需求。
• 已验证对大鼠表皮生长因子（EGF）类似物无显著交叉反应，背景噪声低。
• 完整的一体化试剂盒，包含从样本加样到终止反应的全部关键试剂，降低实验变量。

组件清单

每套试剂盒均配备以下项目（按包装数量计）：

• Rat EGF Microplate
• Rat EGF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat EGF Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

实验步骤概览

1. 将预包被的微孔板加入样本或标准品，37 °C孵育30 min。
2. 洗板去除未结合组分。
3. 加入生物素化检测抗体，继续37 °C孵育30 min。
4. 洗板后加入链霉亲和素‑HRP复合物。
5. 再次洗涤后加入TMB底物，颜色显现后加终止液。
6. 使用酶标仪在450 nm读取OD值，依据标准曲线定量EGF浓度。

使用提示 & 注意事项

• 请勿混用不同批号或已超过有效期的试剂。
• 使用前所有试剂需在室温下平衡30 分钟。
• 实验中建议使用一次性吸头，防止交叉污染。
• 未使用的板条应立即放回原包装，密封后置于4 °C保存。
• 混匀是保证可靠结果的关键，推荐低速振荡或每10 分钟手动摇匀。
• 建议对每个样品或标准进行双孔或三孔复测，以提升数据可信度。

存储与运输

存储建议：未开封的试剂盒请避光置于2‑8 °C，保质期24个月；开封后请参照说明书分别保存各组分。

运输条件：使用蓝色冰袋冷链运输，确保全程温度控制。

警告声明

本产品仅限科研使用，非临床诊断或人用药品。使用本试剂盒进行的任何专利或许可证相关操作，需自行承担风险与责任。

背景信息

表皮生长因子（EGF）是一种小分子多肽，能够通过与EGFR结合激活细胞增殖、分化与修复通路。EGF在口腔黏膜、胃肠道以及多种组织中发挥保护与再生作用；其异常信号常与某些癌症的受体突变或功能异常相关。准确定量大鼠来源的EGF，有助于阐明其在细胞信号传导、组织修复及疾病模型中的功能。

规格与报价

规格	单价（人民币）	交付周期
48 试剂盒	1,598.0 元	期货（5 天内发货）
96 试剂盒	2,558.0 元	期货（5 天内发货）

技术参数

• 检测指标：EGF
• 分子：EGF
• 检测范围：15.63 pg/mL – 1,000 pg/mL
• 灵敏度：15.63 pg/mL
• 检测方法：夹心ELISA

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat EGF ELISA Kit (KTE3074-3)

Product Overview

Chinese Name: Rat Epidermal Growth Factor ELISA Kit
English Name: Rat EGF ELISA Kit
Catalog No.: KTE3074-3
Applicable Species: Rat
Assay Type: ELISA (sandwich format)
Sample Types: Cell culture supernatant, serum, plasma

Core Technology and Advantages

• Based on EliKine™ dual‑antibody sandwich format, providing ultra‑high sensitivity (15.63 pg/mL) and specificity.
• Detection range spans 15.63 pg/mL – 1,000 pg/mL, meeting quantification needs from low to moderate concentrations.
• Validated to exhibit no significant cross‑reactivity with rat EGF homologs, with low background.
• Comprehensive integrated kit containing all critical reagents from sample addition to reaction termination, reducing experimental variability.

Component List

Each kit includes the following items (per package quantity):

• Rat EGF Microplate
• Rat EGF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat EGF Detection Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Assay Procedure Overview

1. Add samples or standards to the pre‑coated microplate and incubate at 37 °C for 30 min.
2. Wash the plate to remove unbound components.
3. Add biotinylated detection antibody and incubate at 37 °C for 30 min.
4. After washing, add streptavidin‑HRP conjugate.
5. Wash again, add TMB substrate, and after color development add stop solution.
6. Read absorbance at 450 nm using a microplate reader and quantify EGF concentration using the standard curve.

Usage Tips & Precautions

• Do not mix reagents from different lots or those past their expiration date.
• Allow all reagents to equilibrate at room temperature for 30 min before use.
• Use disposable tips during the assay to prevent cross‑contamination.
• Unused plate strips should be returned to the original packaging, sealed, and stored at 4 °C.
• Mixing is critical for reliable results; recommend low‑speed vortexing or manual inversion every 10 minutes.
• It is recommended to run each sample or standard in duplicate or triplicate to enhance data reliability.

Storage and Shipping

Storage Recommendation: Store unopened kits protected from light at 2‑8 °C, with a shelf life of 24 months; after opening, follow the manual for individual component storage.

Shipping Conditions: Ship on cold chain using blue ice packs to ensure temperature control throughout transit.

Warning Statement

This product is intended for research use only and is not for clinical diagnosis or human therapeutic applications. Any patent or license‑related activities performed with this kit are undertaken at the user’s own risk and responsibility.

Background Information

Epidermal Growth Factor (EGF) is a small polypeptide that activates cellular proliferation, differentiation, and repair pathways by binding to EGFR. EGF exerts protective and regenerative effects in oral mucosa, the gastrointestinal tract, and various other tissues; aberrant signaling is frequently associated with receptor mutations or functional dysregulation in certain cancers. Accurate quantification of rat‑derived EGF facilitates elucidation of its role in cell signaling, tissue repair, and disease models.

Specifications and Pricing

Specification	Unit Price (CNY)	Delivery Lead Time
48‑well Kit	¥1,598.0	Spot (delivery within 5 days)
96‑well Kit	¥2,558.0	Spot (delivery within 5 days)

Technical Specifications

• Analyte: EGF
• Molecule: EGF
• Detection Range: 15.63 pg/mL – 1,000 pg/mL
• Sensitivity: 15.63 pg/mL
• Assay Method: Sandwich ELISA

Note: The above information may change due to product optimizations or upgrades; for the most current details, please contact Yakein Corporation.