骨保护素酶联免疫试剂盒(人) Osteoprotegerin

Human Osteoprotegerin (OPG) ELISA Kit (KTE3055‑3)

英文名称：Human Osteoprotegerin/OPG ELISA Kit

中文名称：骨保护素酶联免疫试剂盒(人) Osteoprotegerin/OPG ELISA KIT

货号：KTE3055‑3

反应物种：人（Human）

产品概述

本套装基于EliKine™ 双抗体夹心技术，专为定量检测细胞培养上清、血清或血浆中的人骨保护素（OPG）设计。OPG 是调节破骨细胞活性的关键细胞因子，近年来被广泛研究于骨代谢、心血管疾病及糖尿病相关并发症的机制探索中。该试剂盒为科研实验室提供了一种灵敏、特异的定量手段，帮助研究者在蛋白表达、信号通路以及药物干预等项目中获取可靠的定量数据。

适用领域

• 蛋白类分析 – 细胞因子、受体及其相互作用研究
• ELISA 应用 – 体外样本定量、药理筛选、机制验证
• 细胞分析平台 – 细胞培养上清、血清/血浆样本检测
• 疾病模型研究 – 骨代谢、心血管疾病、代谢综合征等

检测原理

采用双抗体夹心法：

1. 预包被抗人 OPG 抗体的酶标板捕获样本中的 OPG。
2. 加入生物素化的检测抗体形成二抗复合物。
3. 链霉亲和素‑HRP 与生物素特异结合，形成可视化酶复合物。
4. HRP 催化 TMB 底物显色后，加止水平终止反应并在 450 nm 测定 OD 值。
5. OD 与标准曲线对应，计算样本中 OPG 的浓度。

关键性能指标

• 检测范围：62.5 pg/mL – 4,000 pg/mL
• 灵敏度（检测下限）：62.5 pg/mL
• 样本类型：细胞培养上清、血清、血浆
• 特异性：未检出显著交叉反应或背景干扰
• 检测方法：酶联免疫吸附（ELISA）——夹心法

试剂盒组成

• Human OPG Microplate（预包被）
• Human OPG Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human OPG Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用注意事项

• 不同批号或已过期的试剂不能混用。
• 使用前请在室温（≈25 °C）平衡 30 分钟。
• 实验过程请使用一次性滤头，防止交叉污染。
• 未用完的板必须立即封回包装，放入 4 °C 冰箱保存。
• 建议使用低速振荡器或每 10 分钟手动轻摇一次，以确保充分混匀。
• 样品及标准建议采用双孔或三孔复测，以提高结果可靠性。

储存与运输

• 未开封试剂盒：避光保存于 2‑8 °C，保质期 24 个月。
• 启用后：请依据说明书分别存放各组分（多数需 2‑8 °C，部分可室温短期保存）。
• 运输条件：使用蓝冰（冷藏）包装，确保全程冷链。

法规与警示

本产品仅供科研使用，不适用于人类临床诊断。使用本试剂盒进行的任何实验应遵守当地法规和伦理要求。我们不对因使用本产品而产生的专利侵权或其他合规风险承担责任。

规格 & 价格

规格	单价（人民币）	交货期
48 T	1,598.0 元	期货（5 天内发货）
96 T	2,558.0 元	期货（5 天内发货）

参考信息

• 基因/蛋白 ID：暂无
• 别名：Osteoprotegerin / OPG，TNFRSF11B
• 检测指标：OPG 蛋白浓度

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Osteoprotegerin (OPG) ELISA Kit (KTE3055‑3)

English Name: Human Osteoprotegerin/OPG ELISA Kit

Chinese Name: Osteoprotegerin ELISA Kit (Human) Osteoprotegerin/OPG ELISA KIT

Catalog Number: KTE3055‑3

Reactivity: Human

Product Overview

This kit is based on the EliKine™ double antibody sandwich technology and is specifically designed for the quantitative detection of Human Osteoprotegerin (OPG) in cell culture supernatants, serum, or plasma. OPG is a key cytokine regulating osteoclast activity and has been extensively studied in recent years in the exploration of mechanisms related to bone metabolism, cardiovascular diseases, and diabetes-related complications. This kit provides a sensitive and specific quantitative method for research laboratories, aiding researchers in obtaining reliable quantitative data for projects involving protein expression, signaling pathways, and drug interventions.

Applications

• Protein Analysis – Study of cytokines, receptors, and their interactions
• ELISA Applications – In vitro sample quantification, pharmacological screening, mechanism validation
• Cellular Analysis Platform – Detection of cell culture supernatants, serum/plasma samples
• Disease Model Research – Bone metabolism, cardiovascular diseases, metabolic syndrome, etc.

Assay Principle

Utilizes the Double Antibody Sandwich Method:

1. The pre-coated anti-human OPG antibody microplate captures OPG from the sample.
2. A biotinylated detection antibody is added to form a secondary antibody complex.
3. Streptavidin-HRP binds specifically to biotin, forming a visible enzyme complex.
4. After HRP catalyzes the TMB substrate to develop color, the stop solution is added to terminate the reaction, and the OD value is measured at 450 nm.
5. The OD value corresponds to the standard curve to calculate the concentration of OPG in the sample.

Key Performance Indicators

• Detection Range: 62.5 pg/mL – 4,000 pg/mL
• Sensitivity (Lower Detection Limit): 62.5 pg/mL
• Sample Types: Cell culture supernatant, serum, plasma
• Specificity: No significant cross-reactivity or background interference detected
• Assay Method: Enzyme-Linked Immunosorbent Assay (ELISA) – Sandwich Method

Kit Components

• Human OPG Microplate (pre-coated)
• Human OPG Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human OPG Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Precautions for Use

• Do not mix reagents from different lots or expired reagents.
• Allow all components to equilibrate to room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Unused wells must be resealed immediately and stored at 4 °C.
• Use a low-speed shaker or gently shake manually every 10 minutes to ensure thorough mixing.
• It is recommended to test samples and standards in duplicate or triplicate to improve result reliability.

Storage and Shipping

• Unopened Kit: Store protected from light at 2-8 °C. Shelf life is 24 months.
• After Opening: Store components separately according to the instructions (most require 2-8 °C, some can be stored at room temperature for short periods).
• Shipping Conditions: Shipped with blue ice (refrigerated) packaging to ensure the cold chain is maintained throughout transit.

Regulations and Warnings

This product is for research use only and is not intended for human clinical diagnosis. Any experiments conducted using this kit should comply with local regulations and ethical requirements. We assume no responsibility for patent infringement or other compliance risks arising from the use of this product.

Specifications & Price

Specification	Unit Price (RMB)	Delivery Time
48 T	1,598.0 Yuan	Future Goods (Ships within 5 days)
96 T	2,558.0 Yuan	Future Goods (Ships within 5 days)

Reference Information

• Gene/Protein ID: Not Available
• Aliases: Osteoprotegerin / OPG, TNFRSF11B
• Detection Target: OPG Protein Concentration

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.